{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T08:09:12Z","timestamp":1783843752071,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T00:00:00Z","timestamp":1777766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T00:00:00Z","timestamp":1777766400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Tuta absoluta<\/jats:italic>\n                      , a major pest of solanaceous crops, has rapidly evolved resistance to spinosyn insecticides, causing significant damage to global crops. Our previous study demonstrated that\n                      <jats:italic>CYP6AB328<\/jats:italic>\n                      plays an important role in the development of spinetoram resistance. However, the direct metabolic role of this detoxification enzyme in spinetoram metabolism remains unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      In this study, we conducted a docking analysis between CYP6AB328 and spinetoram. Our docking analysis showed a good binding affinity between CYP6AB328 and spinetoram, with an energy of less than \u22126.0\u2009kcal\u2009mol\n                      <jats:sup>\u22121<\/jats:sup>\n                      . Metabolomic analysis showed that recombinant CYP6AB328 exhibited substantial metabolic activity toward spinetoram\u2010J (16.62%) and spinetoram\u2010L (12.42%), which were metabolized into two products, N\u2010desmethyl spinetoram J and N\u2010desmethyl spinetoram L, respectively. We also found that laboratory\u2010selected spinetoram\u2010resistant strain of\n                      <jats:italic>T.\u2009absoluta<\/jats:italic>\n                      displayed 34\u2010fold cross\u2010resistance to spinosad, but little to no cross\u2010resistance to imidacloprid (1.26\u2010fold), chlorantraniliprole (1.12\u2010fold), deltamethrin (1.76\u2010fold) and avermectin (1.34\u2010fold. Meanwhile, the expression level of\n                      <jats:italic>CYP6AB328<\/jats:italic>\n                      was positively correlated with spinetoram resistance across four field populations of\n                      <jats:italic>T.\u2009absoluta<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>These findings provide valuable insights into the molecular mechanisms underlying detoxification of spinetoram and support the development of a candidate molecular marker for monitoring spinetoram resistance in the field populations, thereby contributing to improved pest management strategies. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70849","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:00:21Z","timestamp":1777867221000},"page":"7883-7892","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>CYP6AB328<\/scp>\n                    \u2010mediated spinetoram resistance via N\u2010demethylation in\n                    <scp>\n                      <i>Tuta absoluta<\/i>\n                    <\/scp>\n                    : A potential resistance monitoring marker"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6493-6667","authenticated-orcid":false,"given":"Jingang","family":"Xie","sequence":"first","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology Xinjiang University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Furong","family":"Hai","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology Xinjiang University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guorong","family":"Yan","sequence":"additional","affiliation":[{"name":"Crop Research Institute, Xinjiang Academy of Agricultural Sciences  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9796-9309","authenticated-orcid":false,"given":"Xiaoning","family":"Liu","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology Xinjiang University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Liu","sequence":"additional","affiliation":[{"name":"Crop Research Institute, Xinjiang Academy of Agricultural Sciences  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2095-3119(20)63165-3"},{"key":"e_1_2_8_3_1","first-page":"5475","article-title":"Outbreak of the South American tomato leafminer, Tuta absoluta, in the chinese mainland: geographic and potential host range expansion, Pest","volume":"77","author":"Zhang G","year":"2021","journal-title":"Manage Sci"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1127\/entomologia\/2021\/1174"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3931"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-020-01301-y"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1127\/entomologia\/2024\/2362"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1127\/entomologia\/2024\/2452"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2023.169229"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2020.11.004"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c05708"},{"key":"e_1_2_8_12_1","unstructured":"FAO Spinetoramhttp:\/\/www.fao.org\/fileadmin\/templates\/agphome\/documents\/Pests_Pesticides\/JMPR\/Evaluation08\/Spinetoram.pdf. 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