{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:43:26Z","timestamp":1787316206409,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T00:00:00Z","timestamp":1776470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T00:00:00Z","timestamp":1776470400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Several P450 genes in the 20\u2010hydroxyecdysone (20E) signaling pathway are known to critically regulate reproductive development in insects. However, the contribution of other P450 family members to insect reproduction remains poorly characterized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Here, we identified a novel P450 gene,\n                      <jats:italic>CYP12A2<\/jats:italic>\n                      , from the highly polyphagous pest\n                      <jats:italic>Mythimna separata<\/jats:italic>\n                      . Quantitative real\u2010time polymerase chain reaction analysis revealed that\n                      <jats:italic>CYP12A2<\/jats:italic>\n                      was upregulated during sexual maturation and predominantly enriched in the ovaries of 3\u2010day\u2010old female adults. RNA interference (RNAi)\u2010mediated knockdown of\n                      <jats:italic>CYP12A2<\/jats:italic>\n                      severely disrupted ovarian development and downregulated the expression of vitellogenesis\u2010related genes (\n                      <jats:italic>Vg<\/jats:italic>\n                      and\n                      <jats:italic>VgR<\/jats:italic>\n                      ) as well as eggshell formation\u2010related genes (\n                      <jats:italic>CB\u2010Ld329<\/jats:italic>\n                      and\n                      <jats:italic>FCP3C<\/jats:italic>\n                      ), ultimately leading to reduced fecundity. Moreover, silencing of\n                      <jats:italic>CYP12A2<\/jats:italic>\n                      significantly decreased 20E titers and suppressed the expression of key 20E biosynthetic genes (\n                      <jats:italic>CYP307A1<\/jats:italic>\n                      ,\n                      <jats:italic>CYP306A1<\/jats:italic>\n                      ,\n                      <jats:italic>CYP302A1<\/jats:italic>\n                      and\n                      <jats:italic>CYP315A1<\/jats:italic>\n                      ). Importantly, exogenous 20E injection substantially rescued the defects in ovarian development and restored the expression of reproduction\u2010related genes that were impaired by\n                      <jats:italic>CYP12A2<\/jats:italic>\n                      depletion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      These findings indicate that\n                      <jats:italic>CYP12A2<\/jats:italic>\n                      regulates reproduction of\n                      <jats:italic>M. separata<\/jats:italic>\n                      by modulating 20E biosynthesis. Our study uncovers a novel reproductive role for\n                      <jats:italic>CYP12A2<\/jats:italic>\n                      and highlights its potential as a target for RNAi\u2010based pest management strategies. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70853","type":"journal-article","created":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T04:35:28Z","timestamp":1776573328000},"page":"7925-7934","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A novel cytochrome\n                    <scp>P450<\/scp>\n                    gene,\n                    <i>CYP12A2<\/i>\n                    , regulates reproduction of\n                    <i>Mythimna separata<\/i>\n                    by modulating\n                    <scp>20E<\/scp>\n                    biosynthesis"],"prefix":"10.1002","volume":"82","author":[{"given":"Lingling","family":"Li","sequence":"first","affiliation":[{"name":"Guizhou Key Laboratory of Agricultural Biosecurity, Guizhou Branch of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Guizhou Academy of Agricultural Sciences  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenmeng","family":"Li","sequence":"additional","affiliation":[{"name":"Guizhou Key Laboratory of Agricultural Biosecurity, Guizhou Branch of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Guizhou Academy of Agricultural Sciences  Guiyang China"},{"name":"Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, College of Forestry Southwest Forestry University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liao","sequence":"additional","affiliation":[{"name":"Guizhou Key Laboratory of Agricultural Biosecurity, Guizhou Branch of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Guizhou Academy of Agricultural Sciences  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiying","family":"Li","sequence":"additional","affiliation":[{"name":"Guizhou Key Laboratory of Agricultural Biosecurity, Guizhou Branch of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Guizhou Academy of Agricultural Sciences  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changgeng","family":"Dai","sequence":"additional","affiliation":[{"name":"Guizhou Key Laboratory of Agricultural Biosecurity, Guizhou Branch of State Key Laboratory for Biology of Plant Diseases and Insect 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