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However, the specific role of\n                      <jats:italic>LsAtg8<\/jats:italic>\n                      in reproduction and endosymbiont regulation in\n                      <jats:italic>Laodelphax striatellus<\/jats:italic>\n                      has not been fully examined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      In this study, we discovered that\n                      <jats:italic>LsAtg8<\/jats:italic>\n                      is expressed at higher levels in the fat body and\u00a0ovaries of\n                      <jats:italic>L.\u2009striatellus<\/jats:italic>\n                      compared to other autophagy\u2010related genes, regardless of endosymbiont\n                      <jats:italic>Wolbachia<\/jats:italic>\n                      infection status. Knockdown of\n                      <jats:italic>LsAtg8<\/jats:italic>\n                      using RNA interference significantly reduced female fecundity and delayed oocyte development in\n                      <jats:italic>Wolbachia<\/jats:italic>\n                      \u2010infected females, but not in uninfected females. RNA\u2010seq analysis revealed that\n                      <jats:italic>LsAtg8<\/jats:italic>\n                      interference caused significant changes in the fatty acid metabolism pathway in the ovary, and the expression of several genes, including trifunctional enzyme subunit alpha (\n                      <jats:italic>HADHA<\/jats:italic>\n                      ), fatty acid synthase (\n                      <jats:italic>FAS<\/jats:italic>\n                      ) and acyl\u2010CoA delta (11) desaturase (\n                      <jats:italic>FAD\u039411<\/jats:italic>\n                      ), was altered. Additionally,\n                      <jats:italic>LsAtg8<\/jats:italic>\n                      silencing increased\n                      <jats:italic>Wolbachia<\/jats:italic>\n                      levels in embryos and altered its dynamics of density during the development of nymphs without affecting the lifespan or reproductive capacity of the offspring.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      These findings suggest a multifaceted role for\n                      <jats:italic>Atg8<\/jats:italic>\n                      in the host\u2013symbiont system and provide insights into pest management strategies targeting endosymbionts. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70934","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T04:16:09Z","timestamp":1780028169000},"page":"8858-8867","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Silencing\n                    <scp>\n                      <i>LsAtg8<\/i>\n                    <\/scp>\n                    reduces female fecundity and elevates\n                    <i>Wolbachia<\/i>\n                    density in embryos of the rice planthopper\n                    <i>Laodelphax striatellus<\/i>"],"prefix":"10.1002","volume":"82","author":[{"given":"Meng\u2010Ke","family":"Wang","sequence":"first","affiliation":[{"name":"Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing 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