{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T16:41:11Z","timestamp":1786293671575,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      In Brazil,\n                      <jats:italic>Aedes aegypti<\/jats:italic>\n                      is the primary vector of arboviruses such as dengue, chikungunya, yellow fever, and Zika virus, posing a major public health concern, particularly in underdeveloped regions where insecticide resistance and the circulation of highly virulent strains are increasing. In search of alternative and sustainable control strategies, this study evaluated the biolarvicidal potential of compounds from the fruits of\n                      <jats:italic>Melia azedarach<\/jats:italic>\n                      against third\u2010instar\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      larvae, focusing on larvicidal efficacy and associated physiological alterations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      In this study, the crude methanolic extract caused 100% larval mortality at 100\u2009ppm. After liquid\u2013liquid partitioning, the dichloromethane fraction exhibited the highest larvicidal activity (86.0% mortality). Bioassay\u2010guided fractionation led to the isolation of the new compound 17\u2010\n                      <jats:italic>epi<\/jats:italic>\n                      \u2010melianodiol (1), a protolimonoid, which showed relevant larvicidal activity (LC\u2085\u2080\u2009=\u200941.5\u2009ppm; LC\u2089\u2080\u2009=\u200964.7\u2009ppm). Larvae exposed to 1 displayed pronounced morphological alterations, including cuticular damage and extensive vacuolization, as revealed by optical and transmission electron microscopy. In addition, 1 significantly increased reactive oxygen species (ROS) levels in the larval midgut, indicating the occurrence of oxidative imbalance associated with tissue damage.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>\n                      In summary, these combined results suggest that 17\u2010\n                      <jats:italic>epi<\/jats:italic>\n                      \u2010melianodiol is a novel plant\u2010derived larvicide with promising potential, contributing to our understanding of its physiological effects on\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      larvae. \u00a9 2026 The Author(s).\n                      <jats:italic>Pest Management Science<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70968","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T03:46:58Z","timestamp":1779940018000},"page":"9240-9248","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["17\u2010\n                    <scp>\n                      <i>epi<\/i>\n                    <\/scp>\n                    \u2010Melianodiol, a new protolimonoid from\n                    <scp>\n                      <i>Melia azedarach<\/i>\n                    <\/scp>\n                    fruits, exhibits larvicidal activity and is associated with oxidative imbalance and midgut epithelial damage in\n                    <scp>\n                      <i>Aedes aegypti<\/i>\n                    <\/scp>\n                    larvae"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0632-4611","authenticated-orcid":false,"given":"Kethleen Duarte Crespo","family":"Soares","sequence":"first","affiliation":[{"name":"Universidade Estadual do Norte Fluminense Darcy Ribeiro Setor de Qu\u00edmica dos Produtos Naturais, Laborat\u00f3rio de Qu\u00edmica Bio\u2010org\u00e2nica  Campos dos Goytacazes Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9287-9750","authenticated-orcid":false,"given":"Thalya Soares Ribeiro","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Norte Fluminense Darcy Ribeiro Setor de Qu\u00edmica dos Produtos Naturais, Laborat\u00f3rio de Qu\u00edmica Bio\u2010org\u00e2nica  Campos dos Goytacazes Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Aparecida Aride","family":"Bertonceli","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Norte Fluminense Darcy Ribeiro Laborat\u00f3rio de Qu\u00edmica e Fun\u00e7\u00e3o de Prote\u00ednas e Pept\u00eddeos, Centro de Bioci\u00eancias e Biotecnologia  Campos dos Goytacazes Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8827-9864","authenticated-orcid":false,"given":"Renata Rodrigues","family":"da Silva Robaina","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Norte Fluminense Darcy Ribeiro Setor de Qu\u00edmica dos Produtos Naturais, Laborat\u00f3rio de Qu\u00edmica Bio\u2010org\u00e2nica  Campos dos Goytacazes Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia Oliveira","family":"da Rocha","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Norte Fluminense Darcy Ribeiro Laborat\u00f3rio de Biologia Celular e Tecidual, Centro de Bioci\u00eancias e Biotecnologia  Campos dos Goytacazes Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Jos\u00e9 Alves","family":"Lemos","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Norte Fluminense Darcy Ribeiro Laborat\u00f3rio de Biotecnologia, Centro de Bioci\u00eancias e Biotecnologia  Campos dos Goytacazes Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4497-4985","authenticated-orcid":false,"given":"K\u00e1tia Valevski Sales","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Norte Fluminense Darcy Ribeiro Laborat\u00f3rio de Qu\u00edmica e Fun\u00e7\u00e3o de Prote\u00ednas e Pept\u00eddeos, Centro de Bioci\u00eancias e Biotecnologia  Campos dos Goytacazes 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