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Leveraging a comprehensive\n                      <jats:italic>de novo<\/jats:italic>\n                      transcriptome, we investigated developmental gene regulation across larval, pupal, and adult stages and identified essential targets for RNA interference (RNAi)\u2010based intervention.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Stage\u2010resolved transcriptomic analyses revealed extensive transcriptional reprogramming associated with metabolism, detoxification, cuticle biosynthesis, endocrine signaling, and sensory perception. Among these, chitin synthase (\n                      <jats:italic>DaCHS<\/jats:italic>\n                      ) emerged as a critical developmental gene, exhibiting pronounced up\u2010regulation during late larval and pupal stages corresponding to intensive cuticle synthesis. Phylogenetic and structural analyses demonstrated that DaCHS is highly conserved among insects and retains canonical catalytic domains and transmembrane topology. Alpha Fold\u2010based structural modeling and molecular docking confirmed stable interaction of DaCHS with its substrate,\n                      <jats:italic>N<\/jats:italic>\n                      \u2010acetylglucosamine, supporting functional conservation of enzymatic activity. Oral delivery of DaCHS double\u2010stranded RNA induced robust transcript suppression, leading to significant mortality and severe developmental defects, including larval and pupal abnormalities, and adults with disrupted wing and abdominal morphogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      These findings establish\n                      <jats:italic>DaCHS<\/jats:italic>\n                      as an indispensable gene for\n                      <jats:italic>D. abbreviates<\/jats:italic>\n                      development and validate transcriptome\u2010guided RNAi as a powerful framework for target discovery. This work provides a strong molecular foundation for developing RNAi\u2010based strategies that can be integrated into sustainable management programs for citrus root weevil control. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71019","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T11:30:51Z","timestamp":1781695851000},"page":"9845-9869","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>\n                      <i>De novo<\/i>\n                    <\/scp>\n                    transcriptome meta\u2010analysis reveals candidate genes involved in life\u2010stage transitions for\n                    <scp>RNAi<\/scp>\n                    \u2010mediated management of the citrus root weevil (\n                    <scp>\n                      <i>Diaprepes abbreviatus<\/i>\n                    <\/scp>\n  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