{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:14:52Z","timestamp":1789172092380,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>20\u2010hydroxyecdysone (20E) coordinates insect reproductive development through extensive metabolic reprogramming. However, the post\u2010transcriptional mechanisms linking hormonal signaling to carbohydrate metabolism during reproductive maturation remain poorly understood in insect pests.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      We identified a novel 20E\u2013\n                      <jats:italic>miR<\/jats:italic>\n                      \u2010\n                      <jats:italic>2788<\/jats:italic>\n                      \u2013\n                      <jats:italic>Treh1<\/jats:italic>\n                      regulatory axis in\n                      <jats:italic>Galeruca daurica<\/jats:italic>\n                      . Developmental profiling revealed an inverse relationship between\n                      <jats:italic>miR\u20102788<\/jats:italic>\n                      and\n                      <jats:italic>Treh1<\/jats:italic>\n                      expression. Overexpression of\n                      <jats:italic>miR<\/jats:italic>\n                      \u2010\n                      <jats:italic>2788<\/jats:italic>\n                      or RNA interference (RNAi)\u2010mediated silencing of\n                      <jats:italic>Treh1<\/jats:italic>\n                      caused trehalose accumulation, reduced expression of chitin biosynthesis genes, and impaired ovarian development. In females, 20E treatment suppressed\n                      <jats:italic>miR<\/jats:italic>\n                      \u2010\n                      <jats:italic>2788<\/jats:italic>\n                      expression and increased\n                      <jats:italic>Treh1<\/jats:italic>\n                      expression, thereby promoting ovarian maturation. Similar reproductive defects were induced by the trehalase inhibitor validamycin.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      These findings suggest that 20E\u2010 reproductive\n                      <jats:italic>miR\u20102788\u2010Treh1<\/jats:italic>\n                      module contributes, at least in part, to the regulation of reproductive maturation through the \u2010mediate control of trehalose metabolism. The 20E\u2013\n                      <jats:italic>miR<\/jats:italic>\n                      \u2010\n                      <jats:italic>2788<\/jats:italic>\n                      \u2013\n                      <jats:italic>Treh1<\/jats:italic>\n                      axis represents an important metabolic checkpoint linking endocrine signaling with reproductive development and provides potential molecular targets for environmentally friendly pest management strategies. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71037","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T07:27:45Z","timestamp":1781594865000},"page":"10039-10050","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting the\n                    <scp>20E<\/scp>\n                    \u2010responsive\n                    <scp>miR<\/scp>\n                    \u20102788\u2013Treh1 axis disrupts chitin biosynthesis and ovarian development in the grassland pest\n                    <i>Galeruca daurica<\/i>"],"prefix":"10.1002","volume":"82","author":[{"given":"Mingze","family":"Shan","sequence":"first","affiliation":[{"name":"Research Center for Grassland Entomology, Inner Mongolia Agricultural University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Center for Grassland Entomology, Inner Mongolia Agricultural University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haichao","family":"Wang","sequence":"additional","affiliation":[{"name":"Research Center for Grassland Entomology, Inner Mongolia Agricultural University  Hohhot 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