{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:14:56Z","timestamp":1789172096271,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T00:00:00Z","timestamp":1782000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T00:00:00Z","timestamp":1782000000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32572804"],"award-info":[{"award-number":["32572804"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302344"],"award-info":[{"award-number":["32302344"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFE0103400"],"award-info":[{"award-number":["2024YFE0103400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,10]]},"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Octopamine (OA) is an important biogenic amine in insects, and regulates multiple physiological and behavioral processes. Tyrosine decarboxylase (TDC) and tyramine\n \u03b2<\/jats:italic>\n \u2010hydroxylase (T\n \u03b2<\/jats:italic>\n H) are critical enzymes in the biosynthesis of OA. The small brown planthopper\n Laodelphax striatellus<\/jats:italic>\n is a major rice pest and causes serious damage by transmitting plant viruses, such as rice stripe virus (RSV). However, the functions of TDC and T\n \u03b2<\/jats:italic>\n H remain largely unknown in rice planthoppers.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n We cloned\n LsTDC<\/jats:italic>\n and\n LsT\u03b2H<\/jats:italic>\n from\n L. striatellus<\/jats:italic>\n .\n LsTDC<\/jats:italic>\n was significantly enriched in the 4th instar nymphs, and\n LsT\u03b2H<\/jats:italic>\n was highly expressed in eggs.\n LsTDC<\/jats:italic>\n and\n LsT\u03b2H<\/jats:italic>\n were predominantly expressed in the brain. RNA interference (RNAi) targeting\u2010mediated knockdown of\n LsTDC<\/jats:italic>\n and\n LsT\u03b2H<\/jats:italic>\n in female\n L. striatellus<\/jats:italic>\n significantly reduced the fecundity and egg hatchability. Moreover,\n LsTDC<\/jats:italic>\n and\n LsT\u03b2H<\/jats:italic>\n knockdown markedly decreased the transcript levels of\n vitellogenin<\/jats:italic>\n (\n LsVg<\/jats:italic>\n ) in the fat body and ovary, and\n Vg receptor<\/jats:italic>\n (\n LsVgR<\/jats:italic>\n ) in the ovary, as well as changed the expressions of genes involved in juvenile hormone (JH) and 20\u2010hydroxyecdysone (20E) pathways. Additionally, targeting\n LsTDC<\/jats:italic>\n and\n LsT\u03b2H<\/jats:italic>\n significantly reduced the honeydew excretion and body weight of both female and male adults. Notably, the expressions of\n LsTDC<\/jats:italic>\n and\n LsT\u03b2H<\/jats:italic>\n were significantly higher in RSV\u2010viruliferous\n L. striatellus<\/jats:italic>\n , while\n LsTDC<\/jats:italic>\n and\n LsT\u03b2H<\/jats:italic>\n knockdown affected the expressions of capsid protein (\n CP<\/jats:italic>\n ), and vertical transmission rate of RSV.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n These results provide valuable insights into the roles of\n LsTDC<\/jats:italic>\n and\n LsT\u03b2H<\/jats:italic>\n in reproduction, feeding, and virus transmission in\n L. striatellus<\/jats:italic>\n , highlighting their potential as promising targets for developing new pest control strategies. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71041","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T04:35:22Z","timestamp":1782102922000},"page":"10061-10072","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting octopamine synthesis genes\n \n LsTDC<\/i>\n <\/scp>\n and\n \n LsT\u03b2H<\/i>\n <\/scp>\n impairs reproduction, feeding and virus transmission in the small brown planthopper\n \n Laodelphax striatellus<\/i>\n <\/scp>"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7379-8485","authenticated-orcid":false,"given":"Gang","family":"Xu","sequence":"first","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenjia","family":"Shao","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihan","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruhao","family":"Qian","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingshi","family":"Qian","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou 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