{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:14:22Z","timestamp":1789172062234,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Soil\u2010borne\n                      <jats:italic>Fusarium oxysporum<\/jats:italic>\n                      causes destructive wilt in chili (\n                      <jats:italic>Capsicum annuum<\/jats:italic>\n                      L.), yet the critical window for curative chemical intervention remains undefined. This study elucidates early pathogenic mechanisms and optimizes prochloraz application timing for precision disease management.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Fusarium oxysporum<\/jats:italic>\n                      secreted polygalacturonase (PG) and pectin methyl galacturonase (PMG) to compromise root cell integrity, elicit reactive oxygen species (ROS) burst, and reduce root vitality by 78.17%\n                      <jats:italic>versus<\/jats:italic>\n                      healthy controls. Scanning electron microscopy confirmed extensive hyphal colonization.\n                      <jats:italic>In vivo<\/jats:italic>\n                      therapeutic assays demonstrated that prochloraz (80\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) at 2\u2009days post\u2010inoculation (dpi) achieved 73.08% disease control (disease index 29.53), significantly outperforming 4\u2009dpi treatment (44.36% control, disease index 54.81). Mechanistically, prochloraz inhibited conidial germination by 79.15%, suppressed PG and PMG activities by 54.42% and 59.35%, respectively, and attenuated hydrogen peroxide accumulation by 75% in infected roots.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Early\u2010stage prochloraz intervention provides multi\u2010target, mechanism\u2010based curative control of\n                      <jats:italic>Fusarium<\/jats:italic>\n                      wilt by disrupting pathogen establishment and restoring root physiological function. These findings establish a precision timing strategy for integrated disease management in chili production. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71075","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T05:13:26Z","timestamp":1782450806000},"page":"10418-10427","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimizing prochloraz timing for curative control of\n                    <scp>\n                      <i>Fusarium<\/i>\n                    <\/scp>\n                    wilt in chili: a multi\u2010target strategy targeting early root infection"],"prefix":"10.1002","volume":"82","author":[{"given":"Jiaxin","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Green Pesticide Center for R&amp;D of Fine Chemicals of Guizhou University  Guiyang 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