{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T08:04:55Z","timestamp":1784275495236,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1700500"],"award-info":[{"award-number":["2023YFD1700500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      The diamondback moth (\n                      <jats:italic>Plutella xylostella<\/jats:italic>\n                      ) is a globally distributed pest that has developed widespread resistance to multiple classes of insecticides, posing a persistent challenge to cruciferous crop production. Isofluorpyramide is a newly developed isoxazoline insecticide that was created by modifying the structure of fluxametamide. This study aimed to evaluate its insecticidal activity against\n                      <jats:italic>P.\u2009xylostella<\/jats:italic>\n                      and to explore its underlying mode\u2010of\u2010action (MoA).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Leaf\u2010dip bioassays revealed that isofluorpyramide exhibited comparable toxicity to fluxametamide against 2\n                      <jats:sup>nd<\/jats:sup>\n                      \u2010instar\n                      <jats:italic>P.\u2009xylostella<\/jats:italic>\n                      larvae, with a numerically lower lethal concentration 50% (0.036\u2009mg\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) but overlapping 95% confidence intervals (CIs), indicating no significant difference. It retained similar potency against both susceptible and I4790K (ryanodine receptor mutant) strains. In glasshouse pot experiments, isofluorpyramide significantly reduced leaf damage (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05) and provided improved protection over fluxametamide. Field trials confirmed its superior and more persistent control over 14\u2009days. Electrophysiology showed isofluorpyramide inhibited\n                      <jats:italic>Rdl1<\/jats:italic>\n                      subunit\u2010mediated \u03b3\u2010aminobutyric acid (GABA)\u2010induced currents with a numerically lower half\u2010maximal inhibitory concentrations than fluxametamide (overlapping 95% CI, not significant). Molecular docking suggested that enhanced binding affinity stemmed from strengthened van der Waals interactions within the pocket.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Isofluorpyramide is a promising novel insecticide with similar MoA to those of Insecticide Resistance Action Committee Group 30 isoxazoline insecticides (targeting insect GABA\u2010gated chloride channels), and high insecticidal potency. It shows substantial potential for the sustainable management of\n                      <jats:italic>P.\u2009xylostella<\/jats:italic>\n                      and may serve as a crucial tool for managing insecticide resistance in agricultural pest populations. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71121","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T07:17:08Z","timestamp":1784272628000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Insecticidal activity and mode\u2010of\u2010action of the novel insecticide isofluorpyramide against\n                    <i>Plutella xylostella<\/i>"],"prefix":"10.1002","author":[{"given":"Juntao","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Yin","sequence":"additional","affiliation":[{"name":"School of Life Sciences Wuchang University of Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Qin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falong","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University  Wuhan People's Republic of 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China"},{"name":"Department of Crop Protection, Faculty of Agriculture and Natural Resources University of Bakht Alruda  Ed Dueim Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5672-5209","authenticated-orcid":false,"given":"Xiaolei","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health Central China Normal University  Wuhan People's Republic of 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