{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T08:03:07Z","timestamp":1784534587702,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Tobamoviruses infect economically important crops in the Solanaceae and Cucurbitaceae families, causing substantial economic losses. The genus\n                      <jats:italic>Tobamovirus<\/jats:italic>\n                      comprises several highly destructive viruses, such as tobacco mosaic virus (TMV), cucumber green mottle mosaic virus (CGMMV), tomato mosaic virus (ToMV), tomato mottle mosaic virus (ToMMV), pepper mild mottle virus (PMMoV) and tomato brown rugose fruit virus (ToBRFV). Infections caused by these viruses pose serious threats to crop yield and quality, resulting in substantial economic losses. Therefore, rapid and accurate detection of tobamoviruses is crucial for controlling their spread.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      A broad\u2010spectrum detection method for important tobamoviruses was developed based on reverse\u2010transcription recombinase\u2010aided amplification (RT\u2013RAA) combined with lateral flow dipstick (LFD). This RT\u2013RAA\u2013LFD method enables the rapid determination of tobamovirus presence in samples via test strip observation following a 20\u2010min isothermal reaction at 42\u2009\u00b0C. The assay can detect ten virus species in the genus\n                      <jats:italic>Tobamovirus<\/jats:italic>\n                      , namely TMV, ToMV, ToMMV, ToBRFV, PMMoV, CGMMV, cucumber fruit mottle mosaic virus (CFMMV), cucumber mottle virus (CMoV), paprika mild mottle virus (PaMMV) and Rehmannia mosaic virus (ReMV). The detection limits of this method for ToBRFV, CGMMV and PMMoV were approximately 100 times lower than those of a reverse\u2010transcription\u2013polymerase chain reaction. This method exhibits high stability and detection efficiency, with no cross\u2010reactivity against other common viruses infecting Solanaceae and Cucurbitaceae plants.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>This study developed a broad\u2010spectrum RT\u2013RAA\u2013LFD\u2010based detection method for rapid screening of ten tobamoviruses. Our study provides a useful reference for the high\u2010throughput screening of multiple pests in a rapid and simultaneous manner. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71126","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T07:15:27Z","timestamp":1784531727000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid screening of ten tobamoviruses based on reverse\u2010transcription recombinase\u2010aided amplification and lateral flow dipstick"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-2383","authenticated-orcid":false,"given":"Siyuan","family":"Wang","sequence":"first","affiliation":[{"name":"Chinese Academy of Quality and Inspection &amp; Testing  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenxing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Chinese Academy of Quality and Inspection &amp; Testing  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Dong","sequence":"additional","affiliation":[{"name":"Chinese Academy of Quality and Inspection &amp; Testing  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Quality and Inspection &amp; Testing  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1111\/mpp.13229","article-title":"Tomato brown rugose fruit virus: an emerging and rapidly spreading plant RNA virus that threatens tomato production worldwide","volume":"23","author":"Zhang S","year":"2022","journal-title":"Mol Plant Pathol"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1094\/PHYTO-05-22-0167-RVW","article-title":"Plant viruses of agricultural importance: current and future perspectives of virus disease management strategies","volume":"113","author":"Tatineni S","year":"2023","journal-title":"Phytopathology"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"3577","DOI":"10.1016\/j.sjbs.2022.02.027","article-title":"Genetic diversity of cucumber green mottle mosaic virus (CGMMV) infecting cucurbits","volume":"29","author":"Asad Z","year":"2022","journal-title":"Saudi J Biol Sci"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"2641","DOI":"10.1016\/j.jia.2022.07.020","article-title":"Tomato mottle mosaic virus: characterization, resistance gene effectiveness, and quintuplex RT\u2010PCR detection system","volume":"21","author":"Tettey CK","year":"2022","journal-title":"J Integr Agric"},{"key":"e_1_2_10_6_1","volume-title":"Advances in Seed Biology","author":"Dombrovsky A","year":"2017"},{"key":"e_1_2_10_7_1","first-page":"2166","article-title":"To be seen or not to be seen: latent infection by tobamoviruses","volume":"11","author":"Ilyas R","year":"2022","journal-title":"Plants (Basel, Switz)"},{"key":"e_1_2_10_8_1","first-page":"451","article-title":"\u00dcber die Mosaikkrankheit des Tabaks","volume":"32","author":"Mayer A","year":"1942","journal-title":"Landwirtsch Vers\u2010Stn"},{"key":"e_1_2_10_9_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1146\/annurev-virology-100520-014520","article-title":"Tobacco mosaic virus and the history of molecular biology","volume":"9","author":"Creager ANH","year":"2022","journal-title":"Annu Rev Virol"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1111\/j.1744-7348.1965.tb01227.x","article-title":"The epidemiology of tomato mosaic. 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