{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:48:49Z","timestamp":1785908929416,"version":"3.56.0"},"reference-count":49,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Sensitivity to succinate dehydrogenase inhibitor (SDHI) fungicides varies considerably among\n                      <jats:italic>Rhizoctonia<\/jats:italic>\n                      anastomosis groups, yet the molecular basis of this variation remains poorly understood. In this study, the mechanisms underlying the contrasting responses of multinucleate\n                      <jats:italic>Rhizoctonia solani<\/jats:italic>\n                      AG1\u2010IA and binucleate\n                      <jats:italic>Rhizoctonia<\/jats:italic>\n                      AG\u2010Bb to the SDHI fungicide thifluzamide were investigated through an integrated analysis combining fungicide sensitivity assays, structural modeling, molecular docking, molecular dynamics simulations, enzymatic activity measurements and transcriptome profiling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Baseline sensitivity tests revealed a pronounced difference between the two groups. The mean median effective concentration (EC\n                      <jats:sub>50<\/jats:sub>\n                      ) value of AG\u2010Bb isolates (1.99\u2009mg\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) was nearly 28.4\u2010fold higher than that of AG1\u2010IA (0.07\u2009mg\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ), indicating markedly lower sensitivity in the binucleate strain. Structural modeling showed that thifluzamide binds to the ubiquinone\u2010binding pocket formed by SDHB, SDHC and SDHD of the succinate dehydrogenase (SDH) complex. Molecular dynamics simulations further demonstrated a stronger and more stable interaction in AG1\u2010IA, reflected by a substantially lower binding free energy (\u221236.12\u2009kcal\u2009mol\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) compared with AG\u2010Bb (\u221225.76\u2009kcal\u2009mol\n                      <jats:sup>\u22121<\/jats:sup>\n                      ). Consistently, thifluzamide elicited substantially stronger suppression of SDH enzymatic activity in AG1\u2010IA than in AG\u2010Bb. Transcriptomic analysis revealed that genes involved in the ABC transporter pathway were specifically enriched in AG\u2010Bb, suggesting enhanced efflux capacity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      These findings provide mechanistic insight into intrinsic SDHI sensitivity differences among\n                      <jats:italic>Rhizoctonia<\/jats:italic>\n                      groups and contribute to resistance risk assessment for SDHI fungicides. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71153","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:15:01Z","timestamp":1785906901000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular and transcriptomic insights into differential sensitivity of\n                    <i>Rhizoctonia<\/i>\n                    anastomosis groups to the\n                    <scp>SDHI<\/scp>\n                    fungicide thifluzamide"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8282-2766","authenticated-orcid":false,"given":"Yan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, College of Plant Protection Shenyang Agricultural University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, College of Plant Protection Shenyang Agricultural University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Su","sequence":"additional","affiliation":[{"name":"Three\u2010North Shelterbelt Program Promotion Center  Ordos China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songhong","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, College of Plant Protection Shenyang Agricultural University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.70058"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jph.12343"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2019.01.008"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8490"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy14071483"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens13100864"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c03093"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-01-13-0009-RVW"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s42483-024-00254-9","article-title":"Functional analysis of all succinate dehydrogenase subunits in fusarium fujikuroi","volume":"6","author":"Fan M","year":"2024","journal-title":"Phytopathol Res"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c02056"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"2310","DOI":"10.1094\/PDIS-09-21-1993-RE","article-title":"Identification of putative SDHI target\u2010site mutations in SDHB, SDHC and SDHD subunits of Erysiphe necator","volume":"106","author":"Stergiopoulos I","year":"2022","journal-title":"Plant Dis"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8885"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-06-21-0266-R"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof9050545"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS-07-18-1225-RE"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.13470"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12600-017-0600-3"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","DOI":"10.3390\/jof9020214","article-title":"Diversity of mycoviruses present in strains of binucleate Rhizoctonia and multinucleate Rhizoctonia","volume":"9","author":"Li Y","year":"2023","journal-title":"J Fungi"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2010.02.019"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146764"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:EJPP.0000003829.83671.8f"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw054"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky427"},{"key":"e_1_2_8_26_1","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2105-14-S14-S6","article-title":"Homology modeling, molecular docking, and molecular dynamics simulations elucidated \u03b1\u2010fetoprotein binding modes","volume":"14","author":"Shen J","year":"2013","journal-title":"BMC Bioinformatics"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21256"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.7995"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.08.011"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS-12-20-2680-RE"},{"key":"e_1_2_8_31_1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/mSystems.00012-19","article-title":"Comparative transcriptome analysis shows conserved metabolic regulation during production of secondary metabolites in filamentous fungi","volume":"4","author":"Nielsen JC","year":"2019","journal-title":"mSystems"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)69870-4"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5827"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jia.2024.08.017"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.9b00055"},{"key":"e_1_2_8_36_1","first-page":"1","article-title":"Mechanisms of resistance to QoI fungicides in phytopathogenic fungi","volume":"11","author":"Fern\u00e1ndez\u2010Ortu\u00f1o D","year":"2008","journal-title":"Int Microbiol"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/ci500020m"},{"key":"e_1_2_8_38_1","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.01539-22","article-title":"An atypical ABC transporter is involved in antifungal resistance and host interactions in the pathogenic fungus Cryptococcus neoformans","volume":"13","author":"Winski CJ","year":"2022","journal-title":"MBio"},{"key":"e_1_2_8_39_1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/mBio.00457-18","article-title":"A xenobiotic detoxification pathway through transcriptional regulation in filamentous fungi","volume":"9","author":"Sang H","year":"2018","journal-title":"MBio"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2022.10.029"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15188-5"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6155"},{"key":"e_1_2_8_43_1","first-page":"1","article-title":"Molecular mechanisms of succinate dehydrogenase inhibitor resistance in Phytopathogenic fungi","volume":"26","author":"Sang H","year":"2020","journal-title":"Radiat Prot Dosim"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000696"},{"key":"e_1_2_8_45_1","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2023.1273193","article-title":"Multiple and multidrug resistance in Botrytis cinerea: molecular mechanisms of MLR\/MDR strains in Greece and effects of co\u2010existence of different resistance mechanisms on fungicide sensitivity","volume":"14","author":"Sofianos G","year":"2023","journal-title":"Front Plant Sci"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.70191"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy14040666"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12174"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26010262"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c12482"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.71153","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.71153","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.71153","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:15:03Z","timestamp":1785906903000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.71153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":49,"alternative-id":["10.1002\/ps.71153"],"URL":"https:\/\/doi.org\/10.1002\/ps.71153","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,4]]},"assertion":[{"value":"2026-04-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"ps.71153"}}