{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T10:45:33Z","timestamp":1785753933542,"version":"3.56.0"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T00:00:00Z","timestamp":1785628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T00:00:00Z","timestamp":1785628800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Soybean anthracnose caused by\n Colletotrichum truncatum<\/jats:italic>\n is a destructive disease threatening soybean production. Increasing fungicide resistance and concerns over chemical residues highlight the need for new antifungal agents with distinct bioactive scaffolds. This study aims to identify the antifungal metabolite produced by\n Streptomyces paludis<\/jats:italic>\n 13\u20103 and evaluate its potential for soybean anthracnose management\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Bioassay\u2010guided fractionation of the fermented extract of\n S. paludis<\/jats:italic>\n 13\u20103 led to the isolation of oligomycin A, characterized via nuclear magnetic resonance spectroscopy and high\u2010resolution electrospray ionization mass spectrometry. It exhibited half\u2010maximal effective concentration (EC\n 50<\/jats:sub>\n ) values of 0.0173\u2009\u03bcg\u2009mL\n \u22121<\/jats:sup>\n and 0.0811\u2009\u03bcg\u2009mL\n \u22121<\/jats:sup>\n against mycelial growth and conidial germination of\n C. truncatum<\/jats:italic>\n , respectively. Its antifungal potency was approximately 58 times more potent than difenoconazole. At a concentration of 1\u2009\u03bcg\u2009mL\n \u22121<\/jats:sup>\n , oligomycin A achieved 85.85% control efficacy against soybean anthracnose on detached seedlings without inducing phytotoxicity, and inhibited eight tested phytopathogens by 61.69\u201383.39% at 0.1\u2009\u03bcg\u2009mL\n \u22121<\/jats:sup>\n . Fluorescence staining and scanning electron microscopy observations indicated intracellular reactive oxygen species (ROS) accumulation and significant structural damage in\n C. truncatum<\/jats:italic>\n treated with oligomycin A.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n This study provides the first evidence that oligomycin A exhibits strong antifungal activity against\n C. truncatum<\/jats:italic>\n and effectively suppresses soybean anthracnose development in detached seedlings. These findings expand the known antifungal spectrum of oligomycin A and highlight its value as a natural lead compound for the development of antifungal agents against soybean anthracnose. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71177","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T10:00:52Z","timestamp":1785751252000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Oligomycin A from\n \n Streptomyces paludis<\/i>\n <\/scp>\n : potent antifungal activity against\n \n Colletotrichum truncatum<\/i>\n <\/scp>\n and control of soybean anthracnose"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5467-5157","authenticated-orcid":false,"given":"Ziying","family":"Cai","sequence":"first","affiliation":[{"name":"College of Life Sciences Fujian Normal University Fuzhou 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