{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T04:25:34Z","timestamp":1786940734575,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Delphacid planthoppers (Hemiptera: Delphacidae), including the brown planthopper (\n Nilaparvata lugens<\/jats:italic>\n ), pose a significant threat to global rice production, highlighting the urgent need for sustainable and effective biological control agents. Here, we isolated a virulent strain of\n Serratia nematodiphila<\/jats:italic>\n named BPH1 from diseased\n N. lugens<\/jats:italic>\n and characterized its insecticidal activity, genomic features, and virulence\u2010associated phenotypes.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n S. nematodiphila<\/jats:italic>\n BPH1 exhibited median lethal concentrations (LC\n 50<\/jats:sub>\n ) of 5.5\u2009\u00d7\u200910\n 4<\/jats:sup>\n and 2.4\u2009\u00d7\u200910\n 8<\/jats:sup>\n \u2009CFU\/mL for\n N. lugens<\/jats:italic>\n adults and nymphs, respectively. At a concentration of 10\n 8<\/jats:sup>\n \u2009CFU\/mL, the median lethal time (LT\n 50<\/jats:sub>\n ) was approximately 15\u2009h for adults and 45\u2009h for nymphs. Whole\u2010genome sequencing revealed a 5.15\u2009Mb draft genome assembly containing a complete prodigiosin biosynthetic gene cluster and multiple serralysin metalloprotease genes, representing candidate virulence\u2010associated genomic features. The strain exhibited extracellular protease activity and swimming and swarming motility\n in vitro<\/jats:italic>\n , which are commonly associated with bacterial entomopathogenicity. In insecticidal\u2010spectrum assays, the strain caused mortality in two additional delphacid planthoppers,\n Laodelphax striatellus<\/jats:italic>\n and\n Sogatella furcifera<\/jats:italic>\n , and in the mosquito\n Aedes albopictus<\/jats:italic>\n , whereas it showed no pathogenicity toward the green peach aphid\n Myzus persicae<\/jats:italic>\n under the tested conditions.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n These results reveal a virulent\n Serratia<\/jats:italic>\n strain with strong insecticidal activity against rice planthoppers, and highlight genomic and phenotypic features potentially associated with pathogenicity. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71216","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T04:06:31Z","timestamp":1786939591000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and characterization of an entomopathogenic\n Serratia<\/i>\n strain against rice delphacid planthoppers"],"prefix":"10.1002","author":[{"given":"Zhengyu","family":"Zhu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences Zhejiang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqing","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, College of Agriculture Yangtze University Jingzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoqi","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences Zhejiang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Wan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences Zhejiang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7314-377X","authenticated-orcid":false,"given":"Haijun","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences Zhejiang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/156482651203300410"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1138408"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088973"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6915"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c03617"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects15030149"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.09.033"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.49.061802.123310"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fcr.2017.12.008"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252413397"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13580"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8072"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008710"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1915396116"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aspen.2011.09.004"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42452-019-1485-1"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2095-3119(17)61888-4"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2015.10.003"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy10020303"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.6090\/jarq.44.225"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22906-5"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.91774.4"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-19899-7"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8322"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects15090697"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof10040292"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1443651"},{"key":"e_1_2_9_29_1","first-page":"943","article-title":"Entomopathogenic bacteria species and toxins targeting aphids (Hemiptera: Aphididae): a review","volume":"14","author":"Toledo\u2010Hern\u00e1ndez E","year":"2025","journal-title":"Plants (Basel)"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jafr.2022.100376"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/tox348"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13568-022-01442-6"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1703546114"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2021.126956"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.01123-21"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2021.107562"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.129978"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.645701"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.544536"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01908-14"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2016.08.041"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-014-1079-7"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.913113"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2023.127422"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2015.01.018"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2024.105951"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4881"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00017-11"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.2566"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420004045"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27222-0"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1080\/09583157.2015.1089215"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2405"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00173"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)00280-X"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.21307\/pjm-2019-005"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2013-0343"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg159"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.71216","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.71216","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.71216","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T04:06:32Z","timestamp":1786939592000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.71216"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,16]]},"references-count":57,"alternative-id":["10.1002\/ps.71216"],"URL":"https:\/\/doi.org\/10.1002\/ps.71216","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,16]]},"assertion":[{"value":"2026-04-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"ps.71216"}}