{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T05:19:40Z","timestamp":1787548780512,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Ascochyta blight (AB) is a major disease that severely constrains crop yield and quality, capable of causing losses up to 80%. The pathogens responsible for AB are included in a species complex, with\n Didymella pinodella<\/jats:italic>\n being one of the main causal agents. Current methods for detecting\n D. pinodella<\/jats:italic>\n are based on morphological and molecular identification, both of them to be performed under laboratory conditions. To enable rapid on\u2010site diagnosis, we have developed a novel method that integrates recombinase aided amplification (RAA) with a lateral flow dipstick (LFD) for the specific detection of\n D. pinodella<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n By aligning the whole genomes of\n D. pinodella<\/jats:italic>\n and its closely related species, a specific gene fragment was screened and subsequently used as a target sequence for establishing a specific isothermal detection method for\n D. pinodella<\/jats:italic>\n . The RAA\u2010LFD system exhibited high specificity, successfully distinguishing\n D. pinodella<\/jats:italic>\n from closely related species. Both the sensitivity of real\u2010time PCR and the RAA\u2010LFD method for detecting\n D. pinodella<\/jats:italic>\n was 2.13\u2009\u00d7\u200910\n \u22123<\/jats:sup>\n \u2009ng\u2009\u03bcL\n \u22121<\/jats:sup>\n . The RAA\u2010LFD assay successfully produced positive results from pea stems inoculated for 10\u2009days and from artificially infested seed samples, which was consistent with the results obtained by real\u2010time PCR. The total time from DNA extraction to final detection was less than 60\u2009min.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n The method for rapid detection of plant pathogens involves magnetic bead\u2010based DNA extraction, isothermal nucleic acid amplification at 39\u2009\u00b0C, and visual detection using a lateral flow dipstick at room temperature. RAA reagents and primer\u2010probe mixtures can be pre\u2010loaded into microcentrifuge tubes to streamline the on\u2010site operational workflow. This method requires minimal equipment and operational training, demonstrating significant potential for applications in on\u2010site pathogen screening, field surveillance, port quarantine, and controlling the spread of crop fungal pathogens. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71224","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T04:38:53Z","timestamp":1787546333000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A rapid and sensitive method for the detection of\n \n Didymella pinodella<\/i>\n <\/scp>\n in\n \n Pisum sativum<\/i>\n <\/scp>"],"prefix":"10.1002","author":[{"given":"Haipeng","family":"Zhang","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Port Security Inspection Huangpu Customs Technology Center Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanfen","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Port Security Inspection Huangpu Customs Technology Center Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Diversity and Innovative Utilization Institute of Microbiology, Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixia","family":"Feng","sequence":"additional","affiliation":[{"name":"Guangzhou Customs Technology Center Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4043-9558","authenticated-orcid":false,"given":"Rong","family":"Lei","sequence":"additional","affiliation":[{"name":"Chinese Academy of Quality and Inspection & Testing Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/nbu.12399"},{"key":"e_1_2_9_3_1","unstructured":"Economics ABOAR Australian Crop Report: June 2019 No. 190(2019)."},{"key":"e_1_2_9_4_1","unstructured":"Fao F Food and agriculture organization of the United Nations 403(2018).http:\/\/faostat.fao.org."},{"key":"e_1_2_9_5_1","first-page":"44","article-title":"Identification of two species of ascochyta blight from American pea","volume":"30","author":"Zhang Y","year":"2016","journal-title":"Plant Quarant"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.976375"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8010041"},{"key":"e_1_2_9_8_1","article-title":"Didymella pinodella: an important pea root rot pathogen in France to watch out for?","volume":"10","author":"\u0160i\u0161i\u0107 A","year":"2024","journal-title":"Journal of fungi (Basel, Switzerland)"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-015-0798-1"},{"key":"e_1_2_9_10_1","unstructured":"\u0160i\u0161i\u0107A Ba\u0107anovi\u0107\u2010\u0160i\u0161i\u0107JandFinckhMR Proceedings of the 61 Deutsche Pflanzenschutztagung \u201cHerausforderung Pflanzenschutz\u2014Wege in die Zukunft 11\u201314 (University Hohenheim Stuttgart Germany)(2018)."},{"key":"e_1_2_9_11_1","first-page":"207","article-title":"Advances in current research of fungi in genus Didymella","volume":"22","author":"Zhan HX","year":"2024","journal-title":"J Fungal 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