{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:19:40Z","timestamp":1788326380792,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n The oriental fruit fly,\n Bactrocera dorsalis<\/jats:italic>\n (Hendel) (Diptera: Tephritidae), is a destructive pest in the global fruit and vegetable industry. Current management relies heavily on synthetic chemical insecticides, which leads to resistance and environmental risks. Entomopathogenic fungi (EPF) are regarded as promising alternatives to chemical pesticides, but their use against\n B.\u2009dorsalis<\/jats:italic>\n is constrained by stage\u2010dependent efficacy and poor UV persistence.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Twelve fungal isolates were screened against mature larvae, pupae and adults of\n B.\u2009dorsalis<\/jats:italic>\n .\n Metarhizium guizhouense<\/jats:italic>\n WN_M2 showed the most consistent virulence across the three stages, with corrected mortality of 70.2%, 43.6% and 100%, respectively. Three lead isolates,\n M.\u2009guizhouense<\/jats:italic>\n WN_M1 and WN_M2 and\n Beauveria bassiana<\/jats:italic>\n DF_B1, were selected for binary combination assays. Binary combinations enhanced efficacy against subterranean stages. At 1\u2009\u00d7\u200910\n 8<\/jats:sup>\n conidia\u2009mL\n \u22121<\/jats:sup>\n , DF_B1\u2009+\u2009WN_M2 caused 92.86% corrected mortality in mature larvae and 75.31% in pupae. Among the UV\u2010protective additives tested, potassium humate (HA\u2010K) was the most effective, maintaining 86.9% conidial germination at 24\u2009h after 6\u2009h UV\u2010B exposure, compared with 34.6% in the unprotected UV\u2010B control. HA\u2010K also helped preserve fungal pathogenicity against adults under UV\u2010B stress.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n Binary combinations enhanced fungal virulence against subterranean stages, and HA\u2010K improved conidial UV tolerance. These findings provide a promising basis for the further development of fungal biocontrol strategies to sustainably manage this pest. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71247","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T04:28:21Z","timestamp":1788323301000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Improving fungal biocontrol of\n \n Bactrocera dorsalis<\/i>\n <\/scp>\n through binary strain combinations and\n UV<\/scp>\n \u2010protective humate formulations"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-2387-0436","authenticated-orcid":false,"given":"Baihui","family":"Hao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection China 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