{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:17:33Z","timestamp":1787919453295,"version":"build-2784847793"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007565","name":"Citrus Research and Development Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007565","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Citrus root weevil,\n                      <jats:italic>Diaprepes abbreviatus<\/jats:italic>\n                      is one of the most destructive pests of citrus, causing extensive root damage that predisposes trees to secondary infection with phytophthora and substantial yield losses. In the present study, we identified and functionally characterized the\n                      <jats:italic>coatomer subunit beta<\/jats:italic>\n                      gene (\n                      <jats:italic>DaCOPB<\/jats:italic>\n                      ), a core component of the COPI vesicle trafficking machinery responsible for two\u2010way transport between the endoplasmic reticulum and Golgi apparatus.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The full\u2010length\n                      <jats:italic>DaCOPB<\/jats:italic>\n                      transcript was obtained from the\n                      <jats:italic>de novo<\/jats:italic>\n                      transcriptome of\n                      <jats:italic>D. abbreviatus<\/jats:italic>\n                      and exhibited strong evolutionary conservation with insect homologs, including characteristic Armadillo repeat domains associated with protein\u2013protein interactions. Developmental expression analysis revealed that\n                      <jats:italic>DaCOPB<\/jats:italic>\n                      is constitutively expressed across all life stages, suggesting an essential role in insect growth and development. To evaluate its functional importance, dsRNA targeting\n                      <jats:italic>DaCOPB<\/jats:italic>\n                      was orally delivered to larvae at multiple concentrations. RT\u2010qPCR analysis performed 48\u2009h post feeding confirmed significant suppression of\n                      <jats:italic>DaCOPB<\/jats:italic>\n                      transcripts relative to control treatments, demonstrating efficient RNAi\u2010mediated gene silencing. Knockdown of\n                      <jats:italic>DaCOPB<\/jats:italic>\n                      resulted in severe developmental defects, including larval mortality, disrupted pupation, and malformed adults. Regression analysis further revealed a significant dose\u2010dependent correlation between dsRNA exposure and the magnitude of gene suppression and phenotypic effects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      These results demonstrate that\n                      <jats:italic>DaCOPB<\/jats:italic>\n                      is necessary for normal development and survival for\n                      <jats:italic>D. abbreviatus<\/jats:italic>\n                      , highlighting its potential as a promising molecular target for RNAi\u2010based pest management strategies aimed at controlling citrus root weevil populations. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71248","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T11:43:49Z","timestamp":1787917429000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["\u2018Silencing of\n                    <scp>\n                      <i>coatomer subunit beta<\/i>\n                    <\/scp>\n                    disrupts development and causes mortality in the citrus root weevil\n                    <scp>\n                      <i>Diaprepes abbreviatus<\/i>\n                    <\/scp>\n                    \u2019"],"prefix":"10.1002","author":[{"given":"Tejbhan","family":"Saini","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, Citrus Research and Education Center University of Florida  Lake Alfred FL 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