{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T04:20:50Z","timestamp":1787718050196,"version":"build-2784847793"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Pine wilt disease (PWD) caused by\n Bursaphelenchus xylophilus<\/jats:italic>\n is a devastating forest disease worldwide, and there is an urgent need for environmentally friendly biocontrol agents. Cyclic lipopeptides from microbial sources exhibit diverse biological activities, but their nematicidal potential against\n B.\u2009xylophilus<\/jats:italic>\n remains largely unexplored.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n A cyclic lipopeptide, marcenmicin D2, was isolated from the endophytic fungus\n Fusarium<\/jats:italic>\n sp. SSY\u20103. Marcenmicin D2 exhibited potent nematicidal activity against\n B.\u2009xylophilus<\/jats:italic>\n in a concentration\u2010 and time\u2010dependent manner, with a median lethal concentration (LC\n 50<\/jats:sub>\n ) value of 41.6\u2009mg\u2009L\n \u22121<\/jats:sup>\n at 24\u2009h. At 50\u2009mg\u2009L\n \u22121<\/jats:sup>\n , it achieved 100% mortality within 48\u2009h and strongly inhibited egg hatching (>96% at 50\u2009mg\u2009L\n \u22121<\/jats:sup>\n ). The compound rapidly reduced head swing frequency within 3\u2009h, and induced unique morphological abnormalities including aggregation, irregular body bending, coiling and cuticle shrinkage, which were not observed in abamectin\u2010treated nematodes. In treated nematodes, the biochemical evidence of oxidative perturbation (changes in superoxide dismutase, catalase and malondialdehyde) and the transcriptomic signatures, notably the coordinated downregulation of cuticle\u2010related collagens, peroxisomal\/lysosomal pathways, cytochrome P450\u2010dependent xenobiotic metabolism and carbohydrate metabolic genes, are consistent with the multifaceted nature of marcenmicin D2's activity against\n B.\u2009xylophilus<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n Marcenmicin D2 is a potent cyclic lipopeptide nematicide from\n Fusarium<\/jats:italic>\n sp. that acts through disruption of cuticle integrity, impairment of detoxification systems, interference with cellular degradation pathways, and induction of oxidative stress. Its rapid immobilization of\n B.\u2009xylophilus<\/jats:italic>\n , mechanism distinct from that of abamectin and strong egg\u2010hatching inhibition make it a promising lead compound for sustainable PWD management. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71252","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T03:52:37Z","timestamp":1787716357000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nematicidal activity of marcenmicin\n D2<\/scp>\n from\n Fusarium<\/i>\n sp. against\n Bursaphelenchus xylophilus<\/i>"],"prefix":"10.1002","author":[{"given":"Jing","family":"Miao","sequence":"first","affiliation":[{"name":"Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects Huzhou University Huzhou 313000 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui\u2010Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects Huzhou University Huzhou 313000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects Huzhou University Huzhou 313000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Sheng","family":"Gao","sequence":"additional","affiliation":[{"name":"Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects Huzhou University Huzhou 313000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao\u2010Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects Huzhou University Huzhou 313000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1","article-title":"Predicting the global potential distribution of Bursaphelenchus xylophilus using an ecological niche model: expansion trend and the main driving factors","volume":"24","author":"Xiao Y","year":"2024","journal-title":"BMC Ecol Evol"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","DOI":"10.3390\/ijms25137452","article-title":"The Bursaphelenchus xylophilus effector BxNMP1 targets PtTLP\u2010L2 to mediate PtGLU promoting parasitism and virulence in Pinus thunbergii","volume":"25","author":"Yang D","year":"2024","journal-title":"Int J Mol Sci"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","DOI":"10.3390\/f15071253","article-title":"Evaluating the impact of climate change and human activities on 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