{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T04:15:51Z","timestamp":1788322551428,"version":"build-2803163510"},"reference-count":78,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100020014","name":"Embrapa Recursos Gen\u00e9ticos e Biotecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005668","name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Distrito Federal","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007775","name":"Universidade Cat\u00f3lica de Bras\u00edlia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007775","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The root\u2010knot nematode (RKN)\n                    <jats:italic>Meloidogyne incognita<\/jats:italic>\n                    is one of the most important plant\u2010parasitic nematodes (PPN) worldwide.\n                    <jats:italic>M. incognita<\/jats:italic>\n                    secretes a large number of effector proteins that play major roles in parasitism by modulating host susceptibility. In this study, we functionally characterized the\n                    <jats:italic>M. incognita Minc3s00280g09294<\/jats:italic>\n                    (\n                    <jats:italic>Minc09294<\/jats:italic>\n                    ) gene as a putative effector using a host\u2010induced gene silencing (HIGS) approach in transgenic plants. Quantitative expression analyses showed that the\n                    <jats:italic>Minc09294<\/jats:italic>\n                    gene is weakly expressed from eggs through the J4 stage, but is strongly induced in adult females during plant parasitism, indicating a role in the sedentary feeding phase.\n                    <jats:italic>In silico<\/jats:italic>\n                    domain prediction revealed that the encoded Minc09294 protein contains four galectin\/galactose\u2010binding lectin domains, an N\u2010terminal signal peptide for extracellular secretion, a non\u2010cytoplasmic region, and no predicted transmembrane domain, consistent with secretion into host tissues.\n                    <jats:italic>Minc09294<\/jats:italic>\n                    silencing in host plants significantly decreased the number of galls (92.9%), egg masses (93.5%), eggs (79.0%), and the nematode reproduction factor (90.5%) in Minc09294\u2010RNAi lines compared to wild\u2010type plants. Histological analyses further revealed smaller galls and poorly developed giant cells in the\n                    <jats:italic>Minc09294<\/jats:italic>\n                    \u2010RNAi lines. Since the homologous gene\n                    <jats:italic>ME_e1834_066g0351951<\/jats:italic>\n                    in\n                    <jats:italic>Meloidogyne enterolobii<\/jats:italic>\n                    shares high sequence similarity with\n                    <jats:italic>Minc09294<\/jats:italic>\n                    , and consequently with the RNAi target sequence, we also challenged the\n                    <jats:italic>Minc09294<\/jats:italic>\n                    \u2010RNAi lines with\n                    <jats:italic>M. enterolobii<\/jats:italic>\n                    . These\n                    <jats:italic>Minc09294\u2010<\/jats:italic>\n                    RNAi lines exhibited a substantial reduction in the number of galls (up to 53.7%) and egg masses (up to 53.3%), whereas the effects on egg number and reproduction factor were less pronounced. Collectively, these data indicate that the\n                    <jats:italic>Minc09294<\/jats:italic>\n                    in\n                    <jats:italic>M. incognita<\/jats:italic>\n                    and its homolog\n                    <jats:italic>ME_e1834_066g0351951<\/jats:italic>\n                    in\n                    <jats:italic>M. enterolobi<\/jats:italic>\n                    are required for successful parasitism and represent promising effector targets for durable, RNAi\u2010based control of RKNs. \u00a9 2026 The Author(s).\n                    <jats:italic>Pest Management Science<\/jats:italic>\n                    published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                  <\/jats:p>","DOI":"10.1002\/ps.71258","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T03:37:50Z","timestamp":1788320270000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Host\u2010induced silencing of the\n                    <i>Minc09294<\/i>\n                    effector gene impairs\n                    <i>Meloidogyne incognita<\/i>\n                    and\n                    <i>M. Enterolobii<\/i>\n                    infection"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9545-6040","authenticated-orcid":false,"given":"Jo\u00e3o Pedro","family":"Abreu\u2010Sousa","sequence":"first","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"Catholic University of Bras\u00edlia  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thuanne Pires","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7804-1537","authenticated-orcid":false,"given":"Daniele Heloisa","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valdeir Junio Vaz","family":"Moreira","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"},{"name":"Federal University of Brasilia  Brasilia Brazil"},{"name":"Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luanna Pinheiro Alburquerque Freitas","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"Catholic University of Bras\u00edlia  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falia Moura Ros\u00e1rio","family":"Santana","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"Catholic University of Bras\u00edlia  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"},{"name":"Dom Bosco Catholic University  Campo Grande Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lays Antunes","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"Catholic University of Bras\u00edlia  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Eugenia","family":"Lisei\u2010de\u2010Sa","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"},{"name":"Empresa de Pesquisa Agropecu\u00e1ria de Minas Gerais (EPAMIG)  Uberaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deyse Alexandra Rosero","family":"Alpala","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoely Abreu","family":"Reis","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabela Tristan","family":"Louren\u00e7o\u2010Tessutti","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Lima Pepino","family":"Macedo","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Vianna","family":"Morgante","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina Mattar","family":"Silva","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Fernando","family":"Basso","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice","family":"de Almeida\u2010Engler","sequence":"additional","affiliation":[{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"},{"name":"INRAE, Universit\u00e9 C\u00f4te d'Azur, CNRS, ISA  Sophia Antipolis France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8184-9599","authenticated-orcid":false,"given":"Maria Fatima","family":"Grossi\u2010de\u2010Sa","sequence":"additional","affiliation":[{"name":"Embrapa Genetic Resources and Biotechnology (EMBRAPA)  Bras\u00edlia Brazil"},{"name":"Catholic University of Bras\u00edlia  Bras\u00edlia Brazil"},{"name":"INCT PlantStress Biotech EMBRAPA  Bras\u00edlia Brazil"},{"name":"Dom Bosco Catholic University  Campo Grande Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"e_1_2_9_2_1","article-title":"Interactions between soil bacterial diversity and plant\u2010parasitic nematodes in soybean plants","volume":"88","author":"Barros FMDR","year":"2022","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3389\/fpls.2014.00160","article-title":"On the track of transfer cell formation by specialized plant\u2010parasitic nematodes","volume":"5","author":"Rodiuc N","year":"2014","journal-title":"Front Plant Sci"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1111\/mpp.12057","article-title":"Top 10 plant\u2010parasitic nematodes in molecular plant pathology","volume":"14","author":"Jones 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