{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T02:41:07Z","timestamp":1787107267054,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T00:00:00Z","timestamp":1663113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Varroa destructor<\/jats:italic> is a parasitic mite of the honey bee, <jats:italic>Apis mellifera<\/jats:italic>. Its presence in colonies can lead to a collapse within a few years. The use of acaricides has become essential to manage the hive infestation. However, the repeated and possibly incorrect use of acaricide treatments, as <jats:italic>tau<\/jats:italic>\u2010fluvalinate, has led to the development of resistance. The <jats:italic>in vitro<\/jats:italic> phenotypic test allows the proportion of susceptible or resistant individuals to be known following an exposure to an active substance. In <jats:italic>Varroa<\/jats:italic> mites, resistance to <jats:italic>tau<\/jats:italic>\u2010fluvalinate is associated with the presence of mutations at the position 925 of the voltage\u2010gated sodium channel (VGSC).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Here, we compared the results obtained with an <jats:italic>in vitro<\/jats:italic> phenotypic test against <jats:italic>tau<\/jats:italic>\u2010fluvalinate and those obtained with an allelic discrimination assay on 13 treated and untreated <jats:italic>Varroa<\/jats:italic> populations in France. The correlation between the phenotype and the genetic profile rate is found to be 0.89 <jats:italic>Varroa<\/jats:italic> mites having resistant phenotypic profile have a probability of 63% to present the L925V mutation (resistance detection reliability). However, 97% of the <jats:italic>Varroa<\/jats:italic> mites having the susceptible phenotype do not present the L925V mutation (susceptible detection reliability).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The L925V mutation explains most of the resistance to <jats:italic>tau<\/jats:italic>\u2010fluvalinate in <jats:italic>V. destructor<\/jats:italic> in the populations tested. However, other mutations or types of resistance may also be involved to explain the survival of <jats:italic>Varroa<\/jats:italic> mites in the phenotypic test. \u00a9 2022 The Authors. <jats:italic>Pest Management Science<\/jats:italic> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.7126","type":"journal-article","created":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T00:23:03Z","timestamp":1660436583000},"page":"5097-5105","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["<i>Varroa destructor<\/i> resistance to <scp><i>tau<\/i>\u2010fluvalinate<\/scp>: relationship between <i>in vitro<\/i> phenotypic test and <scp>VGSC L925V<\/scp> mutation"],"prefix":"10.1002","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5850-0767","authenticated-orcid":false,"given":"Gabrielle","family":"Almecija","sequence":"first","affiliation":[{"name":"Apinov, Scientific Beekeeping and Training Centre  Lagord France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Schimmerling","sequence":"additional","affiliation":[{"name":"French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Sophia Antipolis Laboratory, Bee Pathology Unit  Sophia Antipolis France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lie","family":"Del Cont","sequence":"additional","affiliation":[{"name":"French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Sophia Antipolis Laboratory, Bee Pathology Unit  Sophia Antipolis 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