{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:17:34Z","timestamp":1788153454141,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin Municipality","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Temperature fluctuations threaten the efficacy of parasitoid wasps used in biological control. Understanding the metabolic basis of thermal adaptation can guide optimized rearing and field release strategies. This study integrated behavioral ecology with untargeted metabolomics (gas chromatography\u2013mass spectrometry (GC\u2013MS) and liquid chromatography\u2013mass spectrometry (LC\u2013MS)) to investigate how\n Chouioia cunea<\/jats:italic>\n , a pupal parasitoid of the fall webworm\n Hyphantria cunea<\/jats:italic>\n , copes with temperature stress.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n Behavioral assays revealed a distinct thermal preference for 21.3\u201324.2\u2009\u00b0C. Low temperature (18\u2009\u00b0C) prolonged development (42.6\u2009days) but increased offspring per host (49\u2009000 wasps), whereas high temperature (29\u2009\u00b0C) accelerated development (13.7\u2009days) at the cost of reduced adult longevity. Metabolomic profiling showed directionally specific reprogramming: cold induced depletion of amino acids and membrane lipids; heat triggered accumulation of thermoprotectants (trehalose, mannitol, dulcitol) and specific amino acids (arginine, threonine). Pathway enrichment identified the aminoacyl\u2010tRNA biosynthesis pathway as a core conserved response under both stresses.<\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n Chouioia cunea<\/jats:italic>\n employs distinct metabolic strategies: resource conservation under cold and thermoprotection under heat. These strategies underpin the temperature\u2010dependent life\u2010history trade\u2010offs observed in this study. From a pest management perspective, maintaining rearing temperatures within 23\u201325\u2009\u00b0C balances colony productivity and wasp quality; prolonged exposure above 28\u2009\u00b0C should be avoided; and low\u2010temperature rearing can be used strategically for colony expansion. The identified stress\u2010responsive metabolites may serve as early quality markers for mass\u2010reared colonies. These findings advance our understanding of metabolic plasticity in beneficial insects and support climate\u2010smart biological control. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71260","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:25:37Z","timestamp":1788150337000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolomics reveals thermal adaptation of the parasitoid wasp\n \n Chouioia cunea<\/i>\n <\/scp>\n via resource conservation and thermoprotection"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5332-2682","authenticated-orcid":false,"given":"Lina","family":"Pan","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity Tianjin Normal University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiping","family":"Hu","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity Tianjin Normal University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyan","family":"Qin","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity Tianjin Normal University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yipeng","family":"Wei","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity Tianjin Normal University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Pan","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity Tianjin Normal University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxin","family":"Yao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity Tianjin Normal University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfang","family":"Gao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity Tianjin Normal University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeyang","family":"Sun","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity Tianjin Normal University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuiqing","family":"Gao","sequence":"additional","affiliation":[{"name":"Co\u2010Innovation Center for Sustainable Forestry in Southern China College of Forestry, Nanjing Forestry University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3684-7453","authenticated-orcid":false,"given":"Penghua","family":"Bai","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection Tianjin Academy of Agricultural Sciences Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gengping","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Entomology Washington State University Pullman WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3230-7678","authenticated-orcid":false,"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity Tianjin Normal University Tianjin 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