{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:51:47Z","timestamp":1791265907084,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n A total of 1000 soil\u2010derived actinomycetes were isolated and screened for antibacterial activity against\n Pectobacterium carotovorum<\/jats:italic>\n subsp\n . carotovorum<\/jats:italic>\n (cabbage soft\u2010rot disease). The most potent isolate,\n KRA705\u201018<\/jats:bold>\n , was selected and UV mutagenesis was performed to improve its bioactivity. The active compound was purified, and its molecular formula and structure were identified through multiple spectroscopic analysis.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n An irradiation time of 90\u2009s was found to be optimal for mutant selection, yielding a 1.09% survival rate. After two successive mutagenesis cycles, the mutant strain (\n Streptomyces<\/jats:italic>\n sp\n .<\/jats:italic>\n KRA0514\u20106<\/jats:bold>\n ) exhibited the strongest and most stable antibacterial activity, consistently achieving over 90% inhibition within 24\u2009h. The culture broth of the mutant achieved complete (100%) cabbage soft rot control at a 250\u2010fold dilution, comparable to oxolinic acid (200\u2009ppm) under glasshouse conditions. Field trials further showed stable activity at the same dilution rates, with control efficacies of more than 70% at 21\u2009days after treatment. Phylogenetic analysis based on 16S rRNA gene sequencing identified the mutant strain as\n Streptomyces<\/jats:italic>\n sp.\n KRA0514\u20106<\/jats:bold>\n , closely related to\n Streptomyces nogalater<\/jats:italic>\n JCM4799T. Bioassay\u2010guided fractionation and purification of culture filtrate using C18 open\u2010column chromatography, Sephadex LH\u201020, preparative high\u2010performance liquid chromatography and hydrophilic interaction liquid chromatography led to the isolation of the active substance HL\u20103.\n In vivo<\/jats:italic>\n glasshouse assays confirmed that purified\n HL\u20103<\/jats:bold>\n provided 100% cabbage soft rot disease control at concentrations >1\u2009ppm. HR\u2010ESI\u2010MS analysis determined the molecular weight of\n HL\u20103<\/jats:bold>\n to be 459\u2009g\u2009mol\n \u22121<\/jats:sup>\n , leading to the identification of its molecular formula as C\n 17<\/jats:sub>\n H\n 29<\/jats:sub>\n N\n 7<\/jats:sub>\n O\n 8<\/jats:sub>\n . Finally, by nuclear magnetic resonance spectroscopic analysis,\n HL\u20103<\/jats:bold>\n was identified as novel streptothricin\u2010like compound containing\n N<\/jats:italic>\n \u2010methylglycine instead of\n \u03b2<\/jats:italic>\n \u2010lysine.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n Overall, UV mutagenesis effectively enhanced the antibacterial potential of the wild strain, and the purified compound,\n HL\u20103<\/jats:bold>\n , exhibited potent activity against cabbage soft rot disease, which can be a promising biocontrol agent for sustainable plant disease management strategies. The results of this study provide new insights into streptothricin structure\u2013activity relationships and suggest the existence of unknown biosynthetic mechanisms of\n S.\u2009nogalater<\/jats:italic>\n . \u00a9 2026 The Author(s).\n Pest Management Science<\/jats:italic>\n published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71282","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:57:58Z","timestamp":1791262678000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UV<\/scp>\n mutagenesis\u2010enhanced\n Streptomyces nogalater<\/i>\n produces a novel streptothricin\u2010like compound with strong activity against cabbage soft rot"],"prefix":"10.1002","author":[{"given":"Young Sook","family":"Kim","sequence":"first","affiliation":[{"name":"Eco\u2010friendly and New Materials Research Center Korea Research Institute of Chemical Technology Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8148-8041","authenticated-orcid":false,"given":"Mirjalol","family":"Umurzokov","sequence":"additional","affiliation":[{"name":"Research Development Division Daeseungbiofarm Co. Ltd Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hae Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Research Development Division Daeseungbiofarm Co. Ltd Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Zhi","family":"Jin","sequence":"additional","affiliation":[{"name":"Microbiome Convergence Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Ae","family":"Kim","sequence":"additional","affiliation":[{"name":"Eco\u2010friendly and New Materials Research Center Korea Research Institute of Chemical Technology Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang Min","family":"Cho","sequence":"additional","affiliation":[{"name":"Research Development Division Daeseungbiofarm Co. 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