{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T17:06:49Z","timestamp":1789405609446,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272572"],"award-info":[{"award-number":["32272572"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32572866"],"award-info":[{"award-number":["32572866"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Discovering novel pesticide targets based on newly synthesized compounds is of great importance for the development of new pesticides and sustainable agricultural. 4\u2010Fluoro\u20102\u2010aryl\u20101\n                      <jats:italic>H<\/jats:italic>\n                      \u2010indole (W1), a 2\u2010arylindole derivative obtained via one\u2010pot synthesis in our previous studies, exhibited potent antifungal activity against\n                      <jats:italic>Botrytis cinerea<\/jats:italic>\n                      B05.10, with a half\u2010maximal effective concentration of 6.78\u2009mg\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      To discover the novel fungicide target, in this study, western blot\u2013drug affinity responsive target stability, surface plasmon resonance and enzymatic assays were used to explore the potential target of W1, and\n                      <jats:italic>B. cinerea<\/jats:italic>\n                      threonyl\u2010tRNA synthetase (BcThrRS) was identified as a direct target of W1 [equilibrium dissociation constant (\n                      <jats:italic>K<\/jats:italic>\n                      <jats:sub>D<\/jats:sub>\n                      ) =\u20095.28\u2009\u03bc\n                      <jats:sc>m<\/jats:sc>\n                      ; half\u2010maximal inhibitory concentration (IC\n                      <jats:sub>50<\/jats:sub>\n                      ) =\u200937.55\u2009\u03bc\n                      <jats:sc>m<\/jats:sc>\n                      ]. Molecular docking and mutagenesis analyses identified Pro256 as a key residue mediating ligand binding, with mutation of this residue substantially reducing target affinity and enzymatic inhibition. Metabolomic profiling suggested that inhibition of BcThrRS by W1 disrupts threonyl\u2010tRNA formation, resulting in defective protein translation, amino acid metabolic imbalance, and downstream perturbation of cellular metabolism.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>These findings indicate that BcThrRS is a promising antifungal target and highlight W1 as a privileged scaffold for the development of fungicides with a novel mode of action. They open up new avenues for the rational design of next\u2010generation antifungals targeting aminoacyl\u2010tRNA synthetases. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71283","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T16:43:34Z","timestamp":1789404214000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of threonyl\u2010\n                    <scp>tRNA<\/scp>\n                    synthetase as a potential fungicidal target of 4\u2010fluoro\u20102\u2010aryl\u2010\n                    <scp>1H<\/scp>\n                    \u2010indole"],"prefix":"10.1002","author":[{"given":"Zhengzhong","family":"Wang","sequence":"first","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding 071001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6968-6088","authenticated-orcid":false,"given":"Jiadong","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding 071001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinda","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding 071001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoshuo","family":"An","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding 071001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinglei","family":"Huo","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding 071001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zexiu","family":"An","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding 071001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lai","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding 071001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding 071001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingqian","family":"Huo","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding 071001 P. R. 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