{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T08:58:39Z","timestamp":1790585919370,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Screening and identifying compounds that modulate oviposition by female\n Spodoptera litura<\/jats:italic>\n moths is a promising strategy for the selective management of this pest. Reverse chemical ecology provides an efficient and target\u2010oriented approach for identifying bioactive semiochemicals by exploiting the functionally relevant olfactory binding proteins.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Scanning electron microscopy revealed the presence of multiple types of sensilla on the ovipositor of female\n S. litura<\/jats:italic>\n , providing morphological support for the selection of ovipositor\u2010expressed olfactory genes as screening targets. In the present study, we used\n SlitPBP3<\/jats:italic>\n , an olfactory gene expressed in the ovipositor of female\n S. litura<\/jats:italic>\n during the calling stage, as a molecular target for screening oviposition\u2010related compounds. Structure\u2010based virtual screening combined with fluorescence competitive binding assays demonstrated that\n SlitPBP3<\/jats:italic>\n showed preferential binding to\n \u03b2<\/jats:italic>\n \u2010ionone among the tested compounds. Site\u2010directed mutagenesis further revealed that residues F12 and F118 are critical for the interaction between\n SlitPBP3<\/jats:italic>\n and\n \u03b2<\/jats:italic>\n \u2010ionone. Functional assays showed that RNA interference\u2010mediated silencing of\n SlitPBP3<\/jats:italic>\n significantly reduced the oviposition preference index of female moths for\n \u03b2<\/jats:italic>\n \u2010ionone\u2010treated substrates. Notably, female moths continued to preferentially oviposit on\n \u03b2<\/jats:italic>\n \u2010ionone\u2010treated substrates even after antennal ablation, indicating that chemosensory perception during oviposition can occur independently of antennal input.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n The present study establishes\n SlitPBP3<\/jats:italic>\n as a practical screening target within a reverse chemical ecology framework and identifies\n \u03b2<\/jats:italic>\n \u2010ionone as an oviposition\u2010stimulatory cue with potential value for developing female\u2010oriented behavioral management strategies against\n S. litura<\/jats:italic>\n . \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71287","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:45:41Z","timestamp":1790228741000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of\n \n \u03b2<\/i>\n <\/scp>\n \u2010ionone as an oviposition preference cue in\n \n Spodoptera litura<\/i>\n <\/scp>\n using\n \n SlitPBP3<\/i>\n <\/scp>\n \u2010based reverse chemical ecology"],"prefix":"10.1002","author":[{"given":"Chunyan","family":"Wu","sequence":"first","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biyun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology Shanxi University Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Gong","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4931-1307","authenticated-orcid":false,"given":"Hangwei","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9575-7019","authenticated-orcid":false,"given":"Yuejun","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology Shanxi University Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8334-3313","authenticated-orcid":false,"given":"Guy","family":"Smagghe","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide Center for R&D of Fine Chemicals of Guizhou University Guiyang China"},{"name":"Institute of Entomology Guizhou University Guiyang China"},{"name":"Department of Biology Vrije Universiteit Brussel (VUB) Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengqi","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide Center for R&D of Fine Chemicals of Guizhou University Guiyang China"},{"name":"Pherobio Semiochemical Institute Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms160922606"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11829-014-9292-5"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-29"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16518-8"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.117.300543"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep07888"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.53706"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2016.00130"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects11010045"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects13050481"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c00749"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41559-017-0314-4"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0201-4"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2020.06.009"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13131"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1127\/entomologia\/2023\/1906"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c05368"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2013.03.016"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10886-010-9902-3"},{"key":"e_1_2_10_22_1","first-page":"754","article-title":"Molecular 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