{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T06:11:06Z","timestamp":1789711866541,"version":"4.0.1"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Plant\u2010parasitic nematodes pose severe and growing threats to global agriculture and forestry. However, the development of effective control strategies is limited by the lack of small\u2010molecule nematicides with well\u2010defined molecular targets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Three undescribed azaphilones, eutylones A\u2013C (\n                      <jats:bold>1<\/jats:bold>\n                      \u2013\n                      <jats:bold>3<\/jats:bold>\n                      ), were isolated from\n                      <jats:italic>Eutypella<\/jats:italic>\n                      sp. NBUF234, a fungus associated with a\n                      <jats:italic>Cinachyrella<\/jats:italic>\n                      sponge collected from the marine mesophotic zone. Their structures were established by spectroscopic analysis, X\u2010ray crystallography, and quantum chemical calculations. Compounds\n                      <jats:bold>2<\/jats:bold>\n                      and\n                      <jats:bold>3<\/jats:bold>\n                      represent the first deflectin\u2010type azaphilones bearing a C\u20105 methyl group and a C8 tetraketone side chain. Compounds\n                      <jats:bold>1<\/jats:bold>\n                      \u2013\n                      <jats:bold>3<\/jats:bold>\n                      showed moderate, dose\u2010dependent activity against\n                      <jats:italic>Bursaphelenchus xylophilus<\/jats:italic>\n                      (PWN) and\n                      <jats:italic>Heterodera glycines<\/jats:italic>\n                      (SCN). In the 72\u2009h analysis, compound\n                      <jats:bold>3<\/jats:bold>\n                      had a lower estimated LC\n                      <jats:sub>50<\/jats:sub>\n                      against PWN than compound\n                      <jats:bold>2<\/jats:bold>\n                      (90.3\n                      <jats:italic>versus<\/jats:italic>\n                      145.8\u2009\u03bcM), whereas compounds\n                      <jats:bold>2<\/jats:bold>\n                      and\n                      <jats:bold>3<\/jats:bold>\n                      had comparable estimated LC\n                      <jats:sub>50<\/jats:sub>\n                      values against SCN (159.1 and 159.6\u2009\u03bcM). Compound\n                      <jats:bold>2<\/jats:bold>\n                      was prioritized for mechanistic profiling based on the preliminary screening results available when those experiments were initiated. Comparative RNA\u2010seq, proteome\u2010wide docking, and RNA interference were used to characterize the response to compound\n                      <jats:bold>2<\/jats:bold>\n                      . These analyses prioritized CAG9107488.1 as a PWN candidate with preliminary functional support, although direct target engagement was not established.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Mesophotic sponge\u2010associated fungi provide structurally novel starting scaffolds for nematicide discovery. The activity ranking was species dependent, and the mechanistic analyses define candidate responses to compound\n                      <jats:bold>2<\/jats:bold>\n                      rather than a shared mechanism for all three compounds. Whether compound\n                      <jats:bold>3<\/jats:bold>\n                      , which had the lower estimated PWN LC\n                      <jats:sub>50<\/jats:sub>\n                      , engages the same response pathway remains to be determined. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71294","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:59:07Z","timestamp":1789711147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nematicidal azaphilones from the mesophotic sponge\u2010associated fungus\n                    <i>Eutypella<\/i>\n                    sp.\n                    <scp>NBUF234<\/scp>"],"prefix":"10.1002","author":[{"given":"Tingting","family":"Wei","sequence":"first","affiliation":[{"name":"Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Health Science Center Ningbo University  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Tian","sequence":"additional","affiliation":[{"name":"Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Health Science Center Ningbo University  Zhejiang China"},{"name":"Hunan University of Medicine  Huaihua China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianjing","family":"Zheng","sequence":"additional","affiliation":[{"name":"Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Health Science Center Ningbo University  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Health Science Center Ningbo University  Zhejiang 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Wu","sequence":"additional","affiliation":[{"name":"Ocean College Zhejiang University  Zhoushan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salman","family":"Khan","sequence":"additional","affiliation":[{"name":"Institute of Drug Discovery and Technology Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haomiao","family":"Ding","sequence":"additional","affiliation":[{"name":"Hwamei College of Life and Health Sciences Zhejiang Wanli University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"He","sequence":"additional","affiliation":[{"name":"Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Health Science Center Ningbo University  Zhejiang China"},{"name":"Ningbo Institute of Marine Medicine Peking University  Ningbo 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