{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T06:04:14Z","timestamp":1789538654543,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Insect salivary proteins play critical roles in modulating plant defenses and facilitating feeding, yet the evolutionary origins and functional mechanisms of most salivary effectors remain poorly understood. This study investigates the evolution and function of multiple inositol polyphosphate phosphatases (MINPPs), the enzymes that specifically expanded in planthopper and bean bug.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Phylogenetic analysis revealed that MINPP genes have undergone lineage\u2010specific multiplication in planthoppers, with up to six paralogs per species. Multiplicated MINPPs exhibited pronounced salivary gland\u2010biased expression, including NlMINPP1b\/2b, LsMINPP1b\/2b, SfMINPP1b\/2b and RpMINPP2b, whereas LsMINPP2c showed ovary\u2010biased expression. RNAi\u2010mediated silencing of salivary gland\u2010biased MINPPs in\n                      <jats:italic>Nilaparvata lugens<\/jats:italic>\n                      ,\n                      <jats:italic>Laodelphax striatellus<\/jats:italic>\n                      and\n                      <jats:italic>Riptortus pedestris<\/jats:italic>\n                      consistently reduced honeydew excretion, decreased fecundity and prolonged developmental duration in treated insects, indicating the importance of these genes. However, when secreted into plants, salivary MINPPs appear to trigger host defense responses. In\n                      <jats:italic>Nicotiana tabacum<\/jats:italic>\n                      plants, transient expression of MINPPs activated callose deposition and suppressed whitefly performance, whereas in rice plants, overexpressing LsMINPP2b likewise reduced planthopper fecundity and honeydew excretion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>MINPPs represent a conserved enzyme family that has diversified through gene duplication to acquire salivary gland\u2010biased expression in a few insects. However, when secreted into plants, MINPPs potentially activate host defenses and reduce insect performance. These findings provide new insights into the evolutionary diversification of insect salivary effectors and highlight MINPPs as potential molecular targets for developing novel pest control strategies. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71307","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:27:26Z","timestamp":1789536446000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>MINPP<\/scp>\n                    : a conserved salivary effector family diversified by gene duplication promotes insect feeding while activating plant defense"],"prefix":"10.1002","author":[{"given":"Xin","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of 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