{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T04:00:51Z","timestamp":1789963251024,"version":"4.0.1"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      The Chinese pear psyllid (\n                      <jats:italic>Cacopsylla chinensis<\/jats:italic>\n                      ), a vector of the bacterial pathogen\n                      <jats:italic>Erwinia amylovora<\/jats:italic>\n                      , displays seasonal polyohenism, transitioning from a summer\u2010form to a winter\u2010form in response to temperature fluctuations. Although the winter\u2010form phenotypes, characterized by enhanced cuticular melanization and thickening, have been extensively documented, the underlying molecular regulatory network remains poorly understood. Given the pivotal biological functions of laccases in insect cuticular melanization and sclerotization, in this research, we systematically investigated the molecular characteristics of the laccase genes\n                      <jats:italic>CcLac1<\/jats:italic>\n                      and\n                      <jats:italic>CcLac2<\/jats:italic>\n                      , as well as their regulatory roles in driving this seasonal polyphenism.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Our results showed that\n                      <jats:italic>CcLac2<\/jats:italic>\n                      was predominantly expressed in the epidermis and markedly upregulated under low\u2010temperature conditions. Functional investigations using RNA interference (RNAi) further revealed that\n                      <jats:italic>CcLac2<\/jats:italic>\n                      plays an essential role in promoting the transition from the summer\u2010 to winter\u2010form by orchestrating cuticular pigment deposition, cuticle thickening, and chitin deposition. In contrast,\n                      <jats:italic>CcLac1<\/jats:italic>\n                      was not significantly involved in low\u2010temperature mediated morphological alterations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      In summary, this study provides solid experimental evidence confirming that\n                      <jats:italic>CcLac2<\/jats:italic>\n                      is critical for seasonal morph transition in\n                      <jats:italic>C. chinensis<\/jats:italic>\n                      , advancing our understanding of the molecular mechanisms underlying arthropod seasonal adaptation and identifying potential molecular targets for precision\u2010based management strategies for this economically important pest. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71318","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T03:06:18Z","timestamp":1789959978000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The laccase gene\n                    <scp>\n                      <i>CcLac2<\/i>\n                    <\/scp>\n                    is essential for the summer\u2010form to winter\u2010form transition in the\n                    <scp>Chinese<\/scp>\n                    pear psyllid"],"prefix":"10.1002","author":[{"given":"Zhixian","family":"Zhang","sequence":"first","affiliation":[{"name":"Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs Guangdong Provincial Key Laboratory of High Technology for Plant Protection  Guangzhou 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