{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:49:03Z","timestamp":1791550143464,"version":"4.3.4"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Cytochrome c peroxidase (Ccp) is a critical enzymatic component in the fungal oxidative stress response and cellular redox homeostasis. However, the biological roles and regulatory mechanism of Ccp in\n                      <jats:italic>Cochliobolus heterostrophus<\/jats:italic>\n                      \u2014a major necrotrophic pathogen responsible for Southern corn leaf blight\u2014remain poorly characterized.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      In this study, we identified and functionally characterized\n                      <jats:italic>ChCCP1<\/jats:italic>\n                      , the gene encoding Ccp in\n                      <jats:italic>C. heterostrophus<\/jats:italic>\n                      . Targeted gene deletion revealed that \u0394\n                      <jats:italic>Chccp1<\/jats:italic>\n                      mutant exhibited significantly reduced conidiation and attenuated virulence on maize leaves, alongside heightened sensitivity to both oxidative and osmotic stress. Protein\u2013protein interaction analyses\u2014including yeast two\u2010hybrid (Y2H) assay and GST pull\u2010down assays\u2014demonstrated a direct physical interaction between ChCcp1 and ChCyc1, a thioredoxin\u2010like protein harboring a conserved peroxiredoxin domain. Subsequent phenotypic and molecular characterization of \u0394Chcyc1 mutants confirmed that\n                      <jats:italic>ChCYC1<\/jats:italic>\n                      is similarly essential for conidiation, full virulence, and stress tolerance. Transcriptomic analysis further showed that deletion of either\n                      <jats:italic>ChCCP1<\/jats:italic>\n                      or\n                      <jats:italic>ChCYC1<\/jats:italic>\n                      lead to significant downregulation of multiple oxidative stress\u2010responsive genes. Notably, both mutant strains displayed enhanced susceptibility to multiple classes of commercial fungicides.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Collectively, these findings establish a functional ChCcp1\u2010ChCyc1 protein complex that coordinately regulates developmental processes (conidiation), pathogenicity, abiotic stress adaption, and fungicide resistance in\n                      <jats:italic>C. heterostrophus<\/jats:italic>\n                      . This work advances our mechanism understanding of fungal pathogenesis and identifies a promising dual\u2010target module for developing novel disease management strategies. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71327","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:51:09Z","timestamp":1791546669000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>ChCcp1<\/scp>\n                    and\n                    <scp>ChCyc1<\/scp>\n                    function as a coordinated redox couple to regulate asexual sporulation, pathogenicity, and oxidative\/osmotic stress adaptation in\n                    <scp>\n                      <i>Cochliobolus heterostrophus<\/i>\n                    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