{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:06:04Z","timestamp":1789977964855,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Abiotic stresses are a major limitation to the efficacy of entomopathogenic fungi in natural ecosystems and in biocontrol applications, yet the mechanisms that confer stress tolerance in\n                      <jats:italic>Metarhizium anisopliae<\/jats:italic>\n                      remain poorly understood. Non\u2010ribosomal peptide synthetases (NRPSs) are key enzymes involved in fungal secondary metabolism and stress responses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>We examined the function of the non\u2010ribosomal peptide synthetase condensation domain in M. anisopliae (MaNRPS_C) by constructing a deletion mutant (\u0394MaNRPS_C), an overexpression strain (OE), and compared them with the wild type (WT). Deletion of MaNRPS_C did not affect pathogenicity toward Sogatella furcifera or Locusta migratoria manilensis, but it markedly reduced conidial germination, sporulation, and tolerance to ultraviolet\u2010B (UV\u2010B) radiation, heat stress, and several chemical stressors. Conversely, the OE strain displayed faster germination, higher conidial yields, and enhanced resistance to these abiotic challenges. Furthermore, MaNRPS_C positively regulated the expression of melanin\u2010biosynthesis genes, resulting in increased pigment accumulation that protected conidia from UV\u2010B\u2010induced damage. In addition, MaNRPS_C upregulated key DNA repair genes (e.g., rad3, rad10) and heat\u2010shock protein genes (e.g., hsp40, hsp70), suggesting enhanced DNA repair capacity and protein homeostasis under stress conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      These results identify\n                      <jats:italic>MaNRPS_<\/jats:italic>\n                      C as a key regulator of abiotic stress tolerance, particularly under UV\u2010B and heat stress in\n                      <jats:italic>M. anisopliae<\/jats:italic>\n                      . Targeted manipulation of this gene may facilitate the development of more robust fungal biopesticides with improved field performance. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71334","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T07:46:55Z","timestamp":1789976815000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <scp>\n                      <i>non\u2010ribosomal peptide synthetase<\/i>\n                    <\/scp>\n                    _C mediates abiotic stress tolerance and modulates\n                    <scp>DNA<\/scp>\n                    damage responses in\n                    <scp>\n                      <i>Metarhizium anisopliae<\/i>\n                    <\/scp>"],"prefix":"10.1002","author":[{"given":"Zhongwei","family":"Chen","sequence":"first","affiliation":[{"name":"School of Life Sciences, Genetic Engineering Research Center Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingchun","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Genetic Engineering Research Center Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujia","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Genetic Engineering Research Center Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5551-703X","authenticated-orcid":false,"given":"Yuxian","family":"Xia","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Genetic Engineering Research Center Chongqing University  Chongqing 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