{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:06:41Z","timestamp":1790053601727,"version":"4.0.1"},"reference-count":69,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      The Colorado potato beetle (CPB),\n                      <jats:italic>Leptinotarsa decemlineata<\/jats:italic>\n                      Say (Coleoptera: Chrysomelidae), is a major pest of potato and other nightshades (Solanaceae). CPB's remarkable ability to rapidly evolve resistance to conventional insecticides has necessitated the development of novel management strategies, including RNA interference (RNAi)\u2010based approaches using insecticidal dsRNA. In previous work, we demonstrated that a dsRNA insecticide targeting\n                      <jats:italic>Actin,<\/jats:italic>\n                      a highly conserved protein essential for cell structure and function, was less effective at low temperatures. Here, we evaluated the extent to which the temperature\u2010dependence of RNAi in CPB varies among geographically distinct populations. Our goal was to quantify genotype \u00d7 environment interactions underlying RNAi efficacy, which could influence regional pest management efficacy and be a pathway to resistance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      We measured gene silencing and mortality for three populations of CPB (Vermont, Kentucky and Oregon) following ingestion of dsRNA targeting\n                      <jats:italic>Actin<\/jats:italic>\n                      at 18 and 25\u2009\u00b0C. At 25\u2009\u00b0C, gene silencing occurred more quickly in the Vermont population but mortality did not vary significantly among populations. ds\n                      <jats:italic>Actin<\/jats:italic>\n                      was more toxic for all populations at 25\u2009\u00b0C relative to 18\u2009\u00b0C. At 18\u2009\u00b0C, the Kentucky population died faster than those from Vermont and Oregon. The estimated consumed dose of insecticidal dsRNA was not an effective predictor of gene silencing or mortality across populations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>RNAi insecticides may function less efficiently at cooler temperatures, and the precise effects of temperature could vary across CPB populations. Although all three populations reached the same levels of mortality at pupation, the rate at which larvae died varied significantly, which could impact the degree of herbivory and affect agricultural yields, particularly if applications occur early in the growing season when the weather is cooler. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71335","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:38:58Z","timestamp":1790051938000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Population\u2010specific variation in temperature\u2010dependent\n                    <scp>RNAi<\/scp>\n                    efficacy in the Colorado potato beetle (\n                    <i>Leptinotarsa decemlineata<\/i>\n                    )"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6445-2474","authenticated-orcid":false,"given":"Mike","family":"Darrington","sequence":"first","affiliation":[{"name":"Department of Entomology University of Kentucky  Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2415-7809","authenticated-orcid":false,"given":"Sophia K","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Entomology University of Kentucky  Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7819-6934","authenticated-orcid":false,"given":"Silvia I","family":"Rondon","sequence":"additional","affiliation":[{"name":"Oregon Integrated Pest Management Center  Corvallis OR USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0509-4902","authenticated-orcid":false,"given":"Zinan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Entomology University of Kentucky  Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9439-5899","authenticated-orcid":false,"given":"Yolanda H","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Plant and Soil Science University of Vermont  Burlington VT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0873-3247","authenticated-orcid":false,"given":"Subba Reddy","family":"Palli","sequence":"additional","affiliation":[{"name":"Department of Entomology University of Kentucky  Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0963-7457","authenticated-orcid":false,"given":"Nicholas M","family":"Teets","sequence":"additional","affiliation":[{"name":"Department of Entomology University of Kentucky  Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1038\/35888","article-title":"Potent and specific genetic interference by double\u2010stranded RNA in Caenorhabditis elegans","volume":"391","author":"Fire A","year":"1998","journal-title":"Nature"},{"key":"e_1_2_7_3_1","first-page":"249","volume-title":"Advances in Insect Physiology","author":"Baum JA","year":"2014"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1038\/35053110","article-title":"Role for a bidentate ribonuclease in the initiation step of RNA interference","volume":"409","author":"Bernstein E","year":"2001","journal-title":"Nature"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/35005107","article-title":"An RNA\u2010directed nuclease mediates post\u2010transcriptional gene silencing in Drosophila cells","volume":"404","author":"Hammond SM","year":"2000","journal-title":"Nature"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1126\/science.1064023","article-title":"Argonaute2, a link between genetic and biochemical analyses of RNAi","volume":"293","author":"Hammond SM","year":"2001","journal-title":"Science"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1126\/science.1102513","article-title":"Argonaute2 is the catalytic engine of mammalian RNAi","volume":"305","author":"Liu J","year":"2004","journal-title":"Science"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","DOI":"10.1002\/arch.21685","article-title":"Inhibitor of apoptosis is an effective target gene for RNAi\u2010mediated control of Colorado potato beetle, Leptinotarsa decemlineata","volume":"104","author":"M\u00e1ximo WPF","year":"2020","journal-title":"Arch Insect Biochem Physiol"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1111\/imb.12942","article-title":"Effective target genes for RNA interference\u2010based management of the cabbage stem flea beetle","volume":"34","author":"Cedden D","year":"2025","journal-title":"Insect Mol Biol"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.tig.2008.03.007","article-title":"Environmental RNA interference","volume":"24","author":"Whangbo JS","year":"2008","journal-title":"Trends Genet"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/eea.12575","article-title":"Implementing the sterile insect technique with RNA interference \u2013 a review","volume":"164","author":"Darrington M","year":"2017","journal-title":"Entomol Exp Appl"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.pestbp.2018.08.005","article-title":"The mysteries of insect RNAi: a focus on dsRNA uptake and transport","volume":"151","author":"V\u00e9lez AM","year":"2018","journal-title":"Pestic Biochem Physiol"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1146\/annurev-ento-011019-025224","article-title":"Mechanisms, applications, and challenges of insect RNA interference","volume":"65","author":"Zhu KY","year":"2020","journal-title":"Annu Rev Entomol"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.peptides.2013.12.014","article-title":"dsRNA degradation in the pea aphid (Acyrthosiphon pisum) associated with lack of response in RNAi feeding and injection assay","volume":"53","author":"Christiaens O","year":"2014","journal-title":"Peptides"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.ibmb.2017.05.008","article-title":"A double\u2010stranded RNA degrading enzyme reduces the efficiency of oral RNA interference in migratory locust","volume":"86","author":"Song HF","year":"2017","journal-title":"Insect Biochem Mol Biol"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ibmb.2016.07.007","article-title":"Variation in RNAi efficacy among insect species is attributable to dsRNA degradation in vivo","volume":"77","author":"Wang K","year":"2016","journal-title":"Insect Biochem Mol Biol"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1093\/icb\/icac137","article-title":"Museum genomics of an agricultural super\u2010pest, the Colorado potato beetle, Leptinotarsa decemlineata (Chrysomelidae), provides evidence of adaptation from standing variation","volume":"62","author":"Cohen ZP","year":"2022","journal-title":"Integr Comp Biol"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","DOI":"10.1093\/molbev\/msac016","article-title":"Genome resequencing reveals rapid, repeated evolution in the Colorado potato beetle","volume":"39","author":"P\u00e9lissi\u00e9 B","year":"2022","journal-title":"Mol Biol Evol"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s12230-008-9052-0","article-title":"Colorado Potato Beetle Resistance to Insecticides","volume":"85","author":"Alyokhin A","year":"2008","journal-title":"Am J Potato Res"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1002\/ps.3826","article-title":"The red queen in a potato field: integrated pest management versus chemical dependency in Colorado potato beetle control","volume":"71","author":"Alyokhin A","year":"2015","journal-title":"Pest Manag Sci"},{"key":"e_1_2_7_21_1","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1093\/jee\/90.5.1144","article-title":"Economic impact of insecticide resistance in the Colorado potato beetle (Coleoptera: Chrysomelidae) on the Michigan potato industry","volume":"90","author":"Grafius E","year":"1997","journal-title":"J Econ Entomol"},{"key":"e_1_2_7_22_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cois.2014.09.011","article-title":"RNA interference in Colorado potato beetle: steps toward development of dsRNA as a commercial insecticide","volume":"6","author":"Palli SR","year":"2014","journal-title":"Curr Opinion Insect Sci"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/S2095-3119(19)62702-4","article-title":"RNA interference in Colorado potato beetle (Leptinotarsa decemlineata): a potential strategy for pest control","volume":"19","author":"Ma MQ","year":"2020","journal-title":"J Integr Agric"},{"key":"e_1_2_7_24_1","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2021.728652","article-title":"First sprayable double\u2010stranded RNA\u2010based biopesticide product targets proteasome subunit Beta Type\u20105 in Colorado potato beetle (Leptinotarsa decemlineata)","volume":"12","author":"Rodrigues TB","year":"2021","journal-title":"Front Plant Sci"},{"key":"e_1_2_7_25_1","unstructured":"EPA Ledprona (CAS# 2433753\u201368\u20103) for Use in or on Potato; Temporary Exemption From the Requirement of a Tolerance(2023)."},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1093\/jee\/toad034","article-title":"Effects of low doses of a novel dsRNA\u2010based biopesticide (Calantha) on the Colorado potato beetle","volume":"116","author":"Pallis S","year":"2023","journal-title":"J Econ Entomol"},{"key":"e_1_2_7_27_1","doi-asserted-by":"crossref","DOI":"10.3390\/agriculture13122283","article-title":"Baseline susceptibility to a novel dsRNA\u2010based insecticide across US populations of Colorado potato beetle","volume":"13","author":"Pallis S","year":"2023","journal-title":"Agriculture (Basel)"},{"key":"e_1_2_7_28_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0197059","article-title":"Development and characterization of the first dsRNA\u2010resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte","volume":"13","author":"Khajuria C","year":"2018","journal-title":"PLoS One"},{"key":"e_1_2_7_29_1","doi-asserted-by":"crossref","first-page":"6523","DOI":"10.1038\/s41598-021-85876-1","article-title":"Selection for high levels of resistance to double\u2010stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata say) using non\u2010transgenic foliar delivery","volume":"11","author":"Mishra S","year":"2021","journal-title":"Sci Rep"},{"key":"e_1_2_7_30_1","doi-asserted-by":"crossref","first-page":"2217","DOI":"10.1007\/s10340-024-01764-3","article-title":"Characterization and potential mechanism of resistance to double\u2010stranded RNA in willow leaf beetle, Plagiodera versicolora","volume":"97","author":"Liao CY","year":"2024","journal-title":"J Pest Sci"},{"key":"e_1_2_7_31_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.pestbp.2020.104569","article-title":"Profiling of RNAi sensitivity after foliar dsRNA exposure in different European populations of Colorado potato beetle reveals a robust response with minor variability","volume":"166","author":"Mehlhorn SG","year":"2020","journal-title":"Pestic Biochem Physiol"},{"key":"e_1_2_7_32_1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/imb.12222","article-title":"The involvement of clathrin\u2010mediated endocytosis and two Sid\u20101\u2010like transmembrane proteins in double\u2010stranded RNA uptake in the Colorado potato beetle midgut","volume":"25","author":"Cappelle K","year":"2016","journal-title":"Insect Mol Biol"},{"key":"e_1_2_7_33_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.pestbp.2025.106783","article-title":"Reduced uptake through clathrin down\u2010regulation is associated with resistance to dsRNA in a population of the Colorado potato beetle (Leptinotarsa decemlineata, say)","volume":"216","author":"Mishra S","year":"2025","journal-title":"Pestic Biochem Physiol"},{"key":"e_1_2_7_34_1","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1038\/nrg2612","article-title":"The genetics of quantitative traits: challenges and prospects","volume":"10","author":"Mackay TFC","year":"2009","journal-title":"Nat Rev Genet"},{"key":"e_1_2_7_35_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002239","article-title":"Cooler temperatures destabilize RNA interference and increase susceptibility of disease vector mosquitoes to viral infection","volume":"7","author":"Adelman ZN","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"e_1_2_7_36_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.molcel.2013.08.023","article-title":"Functionally diverse MicroRNA effector complexes are regulated by extracellular signaling","volume":"52","author":"Wu P\u2010H","year":"2013","journal-title":"Mol Cell"},{"key":"e_1_2_7_37_1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0020-7322(97)00021-4","article-title":"Cytoskeletal elements in insect sensilla","volume":"26","author":"Wolfrum U","year":"1997","journal-title":"Int J Insect Morphol Embryol"},{"key":"e_1_2_7_38_1","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1111\/imb.12996","article-title":"Environmental factors affecting RNAi efficacy: temperature but not plant cultivar influences Colorado potato beetle's response to insecticidal dsRNA","volume":"34","author":"Darrington M","year":"2025","journal-title":"Insect Mol Biol"},{"key":"e_1_2_7_39_1","unstructured":"PaleckiM DurreI ScottAandLawrimoreJ U.S. Climate Normals 2020: U.S. Hourly Climate Normals (1991\u20132020) ed. by Information NNCfE(2021)."},{"key":"e_1_2_7_40_1","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1086\/284913","article-title":"The latitudinal gradient in geographical range: how so many species coexist in the tropics","volume":"133","author":"Stevens GC","year":"1989","journal-title":"Am Nat"},{"key":"e_1_2_7_41_1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1086\/282487","article-title":"Why mountain passes are higher in the tropics","volume":"101","author":"Janzen DH","year":"1967","journal-title":"Am Nat"},{"key":"e_1_2_7_42_1","doi-asserted-by":"crossref","first-page":"4207","DOI":"10.1111\/j.1365-294X.2005.02740.x","article-title":"The voyage of an invasive species across continents: genetic diversity of North American and European Colorado potato beetle populations","volume":"14","author":"Grapputo A","year":"2005","journal-title":"Mol Ecol"},{"key":"e_1_2_7_43_1","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adg0328","article-title":"Evolution of a fatty acyl\u2010CoA elongase underlies desert adaptation in drosophila","volume":"9","author":"Wang Z","year":"2023","journal-title":"Sci Adv"},{"key":"e_1_2_7_44_1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/ps.2048","article-title":"Ingested RNA interference for managing the populations of the Colorado potato beetle, Leptinotarsa decemlineata","volume":"67","author":"Zhu F","year":"2011","journal-title":"Pest Manag Sci"},{"key":"e_1_2_7_45_1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real\u2010time quantitative PCR and the 2(\u2010Delta Delta C(T)) method","volume":"25","author":"Livak KJ","year":"2001","journal-title":"Methods"},{"key":"e_1_2_7_46_1","doi-asserted-by":"crossref","DOI":"10.21105\/joss.01686","article-title":"Welcome to the tidyverse","volume":"4","author":"Wickham H","year":"2017","journal-title":"J Open Source Softw"},{"key":"e_1_2_7_47_1","unstructured":"R Core Team R: A Language and Environment for Statistical Computing(2024)."},{"key":"e_1_2_7_48_1","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-24277-4","volume-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham H","year":"2016"},{"key":"e_1_2_7_49_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v067.i01","article-title":"Fitting linear mixed\u2010effects models using lme4","volume":"67","author":"Bates D","year":"2015","journal-title":"J Stat Softw"},{"key":"e_1_2_7_50_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v082.i13","article-title":"lmerTest package: tests in linear mixed effects models","volume":"82","author":"Kuznetsova A","year":"2017","journal-title":"J Stat Softw"},{"key":"e_1_2_7_51_1","unstructured":"LenthRVandPiaskowskiJ emmeans: Estimated marginal means aka least\u2010squares means. R package(2024)."},{"key":"e_1_2_7_52_1","unstructured":"TherneauT A package for survival analysis in R. R package(2026)."},{"key":"e_1_2_7_53_1","unstructured":"TherneauT coxme: mixed effects Cox models. R package(2024)."},{"key":"e_1_2_7_54_1","volume-title":"An R Companion to Applied Regression","author":"Fox J","year":"2019"},{"key":"e_1_2_7_55_1","doi-asserted-by":"crossref","first-page":"3836","DOI":"10.1002\/ps.6835","article-title":"Toxicity of a novel dsRNA\u2010based insecticide to the Colorado potato beetle in laboratory and field trials","volume":"78","author":"Pallis S","year":"2022","journal-title":"Pest Manag Sci"},{"key":"e_1_2_7_56_1","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1002\/ps.70294","article-title":"Optimizing application timing and frequency of a novel dsRNAi\u2010based insecticide for Colorado potato beetle management","volume":"82","author":"Dimase M","year":"2026","journal-title":"Pest Manag Sci"},{"key":"e_1_2_7_57_1","volume-title":"RNA Interference in Agriculture: Methods, Applications, and Governance","author":"Velez A","year":"2024"},{"key":"e_1_2_7_58_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2024.141530","article-title":"dsRNA\u2010based pesticides: considerations for efficiency and risk assessment","volume":"352","author":"Dalakouras A","year":"2024","journal-title":"Chemosphere"},{"key":"e_1_2_7_59_1","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1093\/jee\/73.3.369","article-title":"Impact of defoliation by the Colorado potato beetle on potato yields","volume":"73","author":"Hare JD","year":"1980","journal-title":"J Econ Entomol"},{"key":"e_1_2_7_60_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0201849","article-title":"Clathrin\u2010dependent endocytosis is associated with RNAi response in the western corn rootworm, Diabrotica virgifera virgifera LeConte","volume":"13","author":"Pinheiro DH","year":"2018","journal-title":"PLoS One"},{"key":"e_1_2_7_61_1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.ibmb.2015.03.009","article-title":"Clathrin\u2010dependent endocytosis plays a predominant role in cellular uptake of double\u2010stranded RNA in the red flour beetle","volume":"60","author":"Xiao D","year":"2015","journal-title":"Insect Biochem Mol Biol"},{"key":"e_1_2_7_62_1","doi-asserted-by":"crossref","first-page":"4776","DOI":"10.1016\/j.jmb.2016.08.013","article-title":"Homeoviscous adaptation and the regulation of membrane lipids","volume":"428","author":"Ernst R","year":"2016","journal-title":"J Mol Biol"},{"key":"e_1_2_7_63_1","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1016\/j.jinsphys.2006.09.007","article-title":"Upregulation of two actin genes and redistribution of actin during diapause and cold stress in the northern house mosquito, Culex pipiens","volume":"52","author":"Kim M","year":"2006","journal-title":"J Insect Physiol"},{"key":"e_1_2_7_64_1","doi-asserted-by":"crossref","DOI":"10.1242\/jeb.249947","article-title":"Freeze\u2010tolerant crickets fortify their actin cytoskeleton in fat body tissue","volume":"228","author":"Oirschot ML","year":"2025","journal-title":"J Exp Biol"},{"key":"e_1_2_7_65_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-72569-4","article-title":"Abiotic and past climatic conditions drive protein abundance variation among natural populations of the caddisfly Crunoecia irrorata","volume":"10","author":"Ebner JN","year":"2020","journal-title":"Sci Rep"},{"key":"e_1_2_7_66_1","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2020.00384","article-title":"The effects of temperature on animal gut microbiomes","volume":"11","author":"Sepulveda J","year":"2020","journal-title":"Front Microbiol"},{"key":"e_1_2_7_67_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.biocontrol.2018.04.010","article-title":"A \u03b2\u2010carotene\u2010amended artificial diet increases larval survival and be applicable in mass rearing of Harmonia axyridis","volume":"123","author":"Sun Y\u2010X","year":"2018","journal-title":"Biol Control"},{"key":"e_1_2_7_68_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1570-7458.1990.tb01376.x","article-title":"Thirty\u2010five years of diapause research with the Colorado potato beetle","volume":"56","author":"Kort CAD","year":"1990","journal-title":"Entomol Exp Appl"},{"key":"e_1_2_7_69_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0345180","article-title":"Interactions among weather and landscape affect Colorado potato beetle population dynamics","volume":"21","author":"Cohen AL","year":"2026","journal-title":"PLoS One"},{"key":"e_1_2_7_70_1","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1603\/0046-225X-31.6.1110","article-title":"Population dynamics of the Colorado potato beetle in an agroecosystem with tomatoes and potatoes with management implications to processing tomatoes","volume":"31","author":"Harding CL","year":"2002","journal-title":"Environ Entomol"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.71335","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.71335","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.71335","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:39:15Z","timestamp":1790051955000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.71335"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,21]]},"references-count":69,"alternative-id":["10.1002\/ps.71335"],"URL":"https:\/\/doi.org\/10.1002\/ps.71335","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,21]]},"assertion":[{"value":"2026-04-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"ps.71335"}}