{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T11:12:10Z","timestamp":1790161930380,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Plant pests such as\n                      <jats:italic>Acyrthosiphon pisum<\/jats:italic>\n                      and\n                      <jats:italic>Bursaphelenchus xylophilus<\/jats:italic>\n                      pose destructive threats to crops and pine forests globally. Widespread application of synthetic pesticides has caused pest resistance and ecological risks, creating an urgent demand for eco\u2010friendly, pesticidal natural products. Endophytic fungi of medicinal plants serve as an untapped reservoir of bioactive natural metabolites, which are a promising resource for excavating novel natural insecticides.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Twenty\u2010three natural derivatives, including 12 tremulane sesquiterpenoids (\n                      <jats:bold>1<\/jats:bold>\n                      \u2013\n                      <jats:bold>12<\/jats:bold>\n                      ), were isolated from\n                      <jats:italic>Preussia<\/jats:italic>\n                      , an endophytic fungus of\n                      <jats:italic>Rhododendron capitatum<\/jats:italic>\n                      . Compounds\n                      <jats:bold>1<\/jats:bold>\n                      \u2013\n                      <jats:bold>4<\/jats:bold>\n                      were previously undescribed, among which preutremutin E (\n                      <jats:bold>1<\/jats:bold>\n                      ) represents the first naturally occurring tremulane carbon\u2010degraded sesquiterpenoid. Their structures were elucidated by spectroscopic data analysis and electronic circular dichroism (ECD) calculations. All compounds were tested for their toxic activities against\n                      <jats:italic>A. pisum<\/jats:italic>\n                      and\n                      <jats:italic>B. xylophilus<\/jats:italic>\n                      , respectively. Compound\n                      <jats:bold>1<\/jats:bold>\n                      exhibited optimal dual pesticidal activity against\n                      <jats:italic>A. pisum<\/jats:italic>\n                      and\n                      <jats:italic>B. xylophilus<\/jats:italic>\n                      , with 72\u2009h LC\n                      <jats:sub>50<\/jats:sub>\n                      (50% Lethal Concentration) values of 74.8 and 65.6\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      , respectively. Mechanistic assays revealed that\n                      <jats:bold>1<\/jats:bold>\n                      inhibited acetylcholinesterase and disrupted detoxification homeostasis in aphids, while inducing ultrastructural deterioration, antioxidant suppression, energy depletion and protein loss in nematodes. Molecular docking suggested\n                      <jats:bold>1<\/jats:bold>\n                      tightly occupies the active pocket of glutathione\n                      <jats:italic>S<\/jats:italic>\n                      \u2010transferase (GST) via dual hydrogen bonds and hydrophobic interactions, blocking the core detoxification function of GST.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      To our knowledge, the present work represents the first systematic report on secondary metabolites and insecticidal potential of endophytic fungi derived from\n                      <jats:italic>R. capitatum<\/jats:italic>\n                      . Notably, compound\n                      <jats:bold>1<\/jats:bold>\n                      , characterized as a novel nor\u2010\n                      <jats:italic>seco<\/jats:italic>\n                      \u2010tremulane\u2010type sesquiterpene, displays potential nematicidal and aphicidal activity via multi\u2010target toxic pathways, indicating that endophytic fungi from\n                      <jats:italic>R. capitatum<\/jats:italic>\n                      serve as a promising and valuable resource for the exploration of innovative natural insecticides. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71337","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T10:26:32Z","timestamp":1790159192000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nor\u2010\n                    <scp>seco<\/scp>\n                    \u2010tremulane sesquiterpenoids from the endophyte fungus of\n                    <scp>\n                      <i>Rhododendron capitatum<\/i>\n                    <\/scp>\n                    : dual aphicidal and nematicidal activity with multi\u2010target toxic mechanisms"],"prefix":"10.1002","author":[{"given":"Yue\u2010Tong","family":"Zhu","sequence":"first","affiliation":[{"name":"Shaanxi Key Laboratory of Natural Products and Chemical Biology, College of Chemistry and Pharmacy Northwest A and F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Natural Products and Chemical Biology, College of Chemistry and Pharmacy Northwest A and F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu\u2010Xia","family":"Liu","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Natural Products and Chemical Biology, College of Chemistry and Pharmacy Northwest A and F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai\u2010Xin","family":"Hou","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Natural Products and Chemical Biology, College of Chemistry and Pharmacy Northwest A and F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Xie","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Natural Products and Chemical Biology, College of Chemistry and Pharmacy Northwest A and F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8788-320X","authenticated-orcid":false,"given":"Chun\u2010Huan","family":"Li","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Natural Products and Chemical Biology, College of Chemistry and Pharmacy Northwest A and F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1360","article-title":"Pest behavioural disruption by plant metabolites: a crop protection challenge","volume":"26","author":"Pavela R","year":"2026","journal-title":"Trends Plant Sci"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1021\/acs.jafc.5c13547","article-title":"Evaluation of insecticidal application potential of glycoside derivatives from Rhododendron capitatum against four aphid species","volume":"74","author":"Zhu YT","year":"2026","journal-title":"J Agric Food Chem"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"4780","DOI":"10.1002\/ps.70592","article-title":"Bibenzyl and flavone glycosides isolated from Dendrobium nobile as plant\u2010derived insecticides 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