{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:14:57Z","timestamp":1790144097421,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Phoma stem canker (PSC) of oilseed rape (\n Brassica napus<\/jats:italic>\n ) is a disease caused by a complex of two related fungal pathogens:\n Plenodomus lingam<\/jats:italic>\n (\n Leptosphaeria maculans<\/jats:italic>\n ) and\n P. biglobosus<\/jats:italic>\n (\n L. biglobosa<\/jats:italic>\n ). While\n P. biglobosus<\/jats:italic>\n is increasingly recognised as an important PSC pathogen, its sensitivity to succinate dehydrogenase inhibitor (SDHI; FRAC group 7) fungicides remains poorly understood.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n In vitro<\/jats:italic>\n sensitivity testing of\n P. biglobosus<\/jats:italic>\n isolates from the UK (2025) and Poland (2024) revealed that resistance to boscalid and fluxapyroxad was predominant in the UK, with 95% (19\/20) of isolates classified as resistant, compared with 6% (1\/17) in Poland. Resistance factors (RFs) were up to ~500 for boscalid and 265 for fluxapyroxad, with strong positive cross\u2010resistance observed (\n R<\/jats:italic>\n 2<\/jats:sup>\n \u2009=\u20090.96). Sequencing of the\n SDHB, SDHC<\/jats:italic>\n and\n SDHD<\/jats:italic>\n subunits in a subset of isolates spanning the observed SDHI sensitivity range revealed\n SDHC<\/jats:italic>\n and\/or\n SDHD<\/jats:italic>\n mutations, while\n SDHB<\/jats:italic>\n remained wild type. Low\u2010resistant and moderate\u2010resistant isolates carried single\n SDH<\/jats:italic>\n mutations, whereas high\u2010resistant isolates carried double\n SDH<\/jats:italic>\n mutations; all sequenced resistant isolates exhibited distinct\n SDH<\/jats:italic>\n mutation profiles. The\n SDH<\/jats:italic>\n mutations identified in\n P. biglobosus<\/jats:italic>\n were all homologous to mutations associated with SDHI resistance in other fungi. In contrast, fluopyram showed partial cross\u2010resistance, with modest sensitivity shifts (RF\u2009\u2264\u20094.5).\n In vitro<\/jats:italic>\n fitness assays revealed no consistent effects of\n SDH<\/jats:italic>\n mutations on mycelial growth or asexual sporulation.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n This study provides the first confirmation of SDHI resistance in\n P. biglobosus<\/jats:italic>\n . These findings have important implications for PSC and resistance management, highlighting the need to target both species within this pathogen complex for sustainable disease control. \u00a9 2026 The Author(s).\n Pest Management Science<\/jats:italic>\n published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71339","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:50:20Z","timestamp":1790142620000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Discovery and characterisation of succinate dehydrogenase inhibitor resistance in\n \n Plenodomus biglobosus<\/i>\n <\/scp>\n (phoma stem canker of oilseed rape)"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9928-1353","authenticated-orcid":false,"given":"Kevin M","family":"King","sequence":"first","affiliation":[{"name":"Rothamsted 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