{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:03:28Z","timestamp":1790226208443,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Phosphine fumigation remains a cornerstone of stored\u2010grain protection, yet widespread resistance in\n                      <jats:italic>Rhyzopertha dominica<\/jats:italic>\n                      threatens its long\u2010term effectiveness. RNA interference (RNAi) through ingestion of double\u2010stranded RNA (dsRNA) offers a sequence\u2010specific mode\u2010of\u2010action (MoA) distinct from conventional insecticides. However, practical development requires identification of orally effective target genes, demonstration of grain\u2010protection benefits, and validation of efficacy in phosphine\u2010resistant populations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Oral delivery of dsRNA produced dose\u2010dependent reductions in adult survival, with survival ranging from 3% to 12% among the five target\u2010gene treatments at 300\u2009ng\u2009\u03bcL\n                      <jats:sup>\u20131<\/jats:sup>\n                      after 10\u2009days of continuous exposure, compared with &gt;90% in the controls. Target transcript levels were reduced to 15\u201348% of control levels following dsRNA exposure, confirming RNAi activity. dsRNA treatment also substantially reduced grain damage, with relative grain weight loss reduced to as low as 8% of the water\u2010treated control at 300\u2009ng\u2009\u03bcL\n                      <jats:sup>\u20131<\/jats:sup>\n                      . Oral RNAi remained effective across populations with different levels of phosphine resistance, with corrected mortality reaching \u224882% by Day 10 in the highly resistant population (RR\u2009=\u20091173.63).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Oral RNAi can suppress\n                      <jats:italic>R.\u2009dominica<\/jats:italic>\n                      populations and reduce grain damage while maintaining efficacy in phosphine\u2010resistant populations under the conditions tested. These findings support RNAi as a complementary MoA that may help diversify management options for stored\u2010grain protection and resistance management. \u00a9 2026 The Author(s).\n                      <jats:italic>Pest Management Science<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71346","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:51:33Z","timestamp":1790225493000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Oral\n                    <scp>RNAi<\/scp>\n                    reduces survival and grain loss in\n                    <i>Rhyzopertha dominica<\/i>\n                    , including phosphine\u2010resistant 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