{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T13:03:01Z","timestamp":1791464581590,"version":"4.3.4"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Bacterial leaf blight (BLB) caused by\n                      <jats:italic>Xanthomonas oryzae<\/jats:italic>\n                      pv.\n                      <jats:italic>oryzae<\/jats:italic>\n                      (\n                      <jats:italic>Xoo<\/jats:italic>\n                      ) seriously endangers global rice production. It is urgent to explore efficient and low\u2010toxic antibacterial candidates for sustainable bacterial blight management.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Herein, 25 indole\u20103\u2010substituted 1,3,4\u2010oxadiazole derivatives were rationally synthesized.\n                      <jats:bold>T2<\/jats:bold>\n                      and\n                      <jats:bold>T8<\/jats:bold>\n                      showed prominent anti\u2010\n                      <jats:italic>Xoo<\/jats:italic>\n                      activity [median effective concentration (EC\n                      <jats:sub>50<\/jats:sub>\n                      )\u2009=\u20092.63 and 1.81\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      ], 19\u201332\u2010fold more potent than thiodiazole copper, with (respectively) 72.35% and 75.68% therapeutic, and 72.64% and 72.33% protective efficacy on rice. Mechanistic studies demonstrated concurrent suppression of EPS\/biofilm formation, induction of reactive oxygen species (ROS\u2010mediated apoptosis in\n                      <jats:italic>Xoo<\/jats:italic>\n                      , and downregulation of virulence and accC\u2010associated genes \u2013 validated by RNA\u2010seq. Molecular dynamics (MD) simulations revealed stable polar anchoring of\n                      <jats:bold>T8<\/jats:bold>\n                      to accC\n                      <jats:italic>\u2010<\/jats:italic>\n                      Arg381\n                      <jats:italic>versus<\/jats:italic>\n                      electrostatic repulsion experienced by\n                      <jats:bold>T2<\/jats:bold>\n                      at accC\u2010Lys250\/Arg23. Biosafety tests on HEK293T cells and silkworms verified their low toxicity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      <jats:bold>T2<\/jats:bold>\n                      and\n                      <jats:bold>T8<\/jats:bold>\n                      were identified as high\u2010efficiency and biosafe anti\u2010\n                      <jats:italic>Xoo<\/jats:italic>\n                      lead compounds. This study provides valuable candidate molecules and a theoretical basis for the development of novel green bactericides against rice bacterial blight. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71348","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:45:26Z","timestamp":1791463526000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synthesis, anti\u2010\n                    <scp>\n                      <i>Xanthomonas oryzae<\/i>\n                    <\/scp>\n                    pv.\n                    <scp>\n                      <i>oryzae<\/i>\n                    <\/scp>\n                    activity and antibacterial mechanism of indole\u20103\u2010substituted\u20101,3,4\u2010oxadiazole 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