{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:58:44Z","timestamp":1790747924424,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171798"],"award-info":[{"award-number":["32171798"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Anoplophora glabripennis<\/jats:italic>\n                      (Asian longhorned beetle, ALB) is a wood\u2010boring forest pest causing tree decline and mortality. The abdominal terminus is essential for oviposition and bears numerous chemosensilla, but its role in gustatory perception and host selection remains unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Eight sensilla were identified on the female abdominal terminus using scanning electron microscopy, including three putative gustatory sensilla. RNA\u2010sequencing of the abdominal terminus identified 30 gustatory receptors (\n                      <jats:italic>AglaGR1<\/jats:italic>\n                      \u2013\n                      <jats:italic>30<\/jats:italic>\n                      ). Reverse transcription quantitative polymerase chain reaction profiling revealed distinct tissue\u2010 and developmental stage expression patterns for 14 detectable AglaGRs. Five AglaGR genes (\n                      <jats:italic>AglaGR16<\/jats:italic>\n                      \/\n                      <jats:italic>24<\/jats:italic>\n                      \/\n                      <jats:italic>25<\/jats:italic>\n                      \/\n                      <jats:italic>28<\/jats:italic>\n                      \/\n                      <jats:italic>30<\/jats:italic>\n                      ) were successfully cloned and encode typical insect gustatory receptor proteins. AglaGR24 and AglaGR25 were putatively sugar\u2010responsive based on phylogenetic inference, although their ligand specificities remain to be directly validated. RNA interference (RNAi) of five AglaGRs produced gene\u2010 and period\u2010specific effects on oviposition\u2010related behaviors, with\n                      <jats:italic>AglaGR30<\/jats:italic>\n                      showing the most consistent effects, including significantly reduced Period II grooving and egg numbers, total egg number, and effective grooving rate (number of egg\u2010bearing grooves\/total number of grooves).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>Although BLAST analysis detected no significant off\u2010target matches, RNAi specificity was not further validated by examining expression of non\u2010target AglaGRs. This study systematically classified the sensilla on the abdominal terminus of ALB and functionally characterized the roles of its gustatory receptors. These findings establish gustatory receptors as key molecular targets for disrupting host selection, providing a scientific basis for developing RNAi\u2010based biopesticides and behavior\u2010modifying compounds that specifically interfere with oviposition decisions. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71358","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:14:17Z","timestamp":1790745257000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gustatory receptors in the abdominal terminus contribute to oviposition decisions in\n                    <i>Anoplophora glabripennis<\/i>"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3217-1320","authenticated-orcid":false,"given":"Qiu\u2010Mei","family":"Zhong","sequence":"first","affiliation":[{"name":"School of Life Sciences, Hebei University 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