{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:04:23Z","timestamp":1790337863624,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Nilaparvata lugens<\/jats:italic>\n                      migrates into the inland via the southwestern rice regions of China (SWRR). If pest control fails in this gateway region, it would pose a serious threat to inland rice production. However, due to the historical lack of systematic insecticide resistance monitoring in SWRR, high\u2010level resistance to commonly used insecticides in field populations is continuously and uncontrollably developing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Compared to the Lab\u2010HZ strain, SWRR populations showed significantly higher resistance to buprofezin and imidacloprid. Specifically, the Wangzhou2024 (WZ24) population from Chongqing exhibited a resistance ratio (RR) of 463.21\u2010fold to buprofezin, while the Dehong2024 (DH24) population from Yunnan showed an RR of 758.75\u2010fold to imidacloprid. In these resistant populations, cytochrome P450 monooxygenase (P450) activity increased by 2.5\u20136.1\u2010fold, and expression of\n                      <jats:italic>CYP427A1<\/jats:italic>\n                      was up\u2010regulated by 3.8\u2010 and 14.4\u2010fold. RNA interference\u2010mediated silencing of\n                      <jats:italic>CYP427A1<\/jats:italic>\n                      increased mortality of approximately 90% following exposure to buprofezin and imidacloprid. Molecular docking revealed that the binding free energies of\n                      <jats:italic>CYP427A1<\/jats:italic>\n                      with buprofezin and imidacloprid were \u22126.1 and \u22126.3 kcal\/mol, respectively, and recombinant\n                      <jats:italic>CYP427A1<\/jats:italic>\n                      could metabolize both insecticides\n                      <jats:italic>in vitro<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      This study provides a systematic assessment of insecticide resistance levels in\n                      <jats:italic>N.\u2009lugens<\/jats:italic>\n                      from SWRR and demonstrates that\n                      <jats:italic>CYP427A1<\/jats:italic>\n                      \u2010mediated metabolism as a key contributor to high\u2010level resistance to buprofezin and imidacloprid in the populations examined. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71359","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T11:56:22Z","timestamp":1790337382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>CYP427A1<\/scp>\n                    \u2010mediated metabolic resistance to buprofezin and imidacloprid in\n                    <i>Nilaparvata lugens<\/i>\n                    from southwestern rice regions of\n                    <scp>China<\/scp>"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7281-6829","authenticated-orcid":false,"given":"Xiangsong","family":"Li","sequence":"first","affiliation":[{"name":"College of Agriculture Sichuan Agricultural University  Chengdu 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Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Agriculture Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhishan","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Agriculture Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Pu","sequence":"additional","affiliation":[{"name":"College of Agriculture Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3328-1094","authenticated-orcid":false,"given":"Xuegui","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agriculture Sichuan Agricultural University  Chengdu 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