{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T14:04:03Z","timestamp":1791813843075,"version":"4.3.4"},"reference-count":56,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      The PIWI\u2010interacting RNA (piRNA) pathway is a conserved transposon silencing mechanism essential for genomic integrity and gametogenesis. While Piwi proteins are known to play essential roles in insect germline development, their functional characteristics remain largely unexplored in agricultural pests. Here, we investigated\n                      <jats:italic>BdPiwi<\/jats:italic>\n                      in\n                      <jats:italic>Bactrocera dorsalis<\/jats:italic>\n                      , a highly invasive pest whose exceptional reproductive capacity underpins its global pest status.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Phylogenetic analysis revealed that BdPiwi is evolutionarily conserved among insects and contains the canonical PAZ and PIWI domains. Spatiotemporal profiling showed ubiquitous expression across developmental stages, with predominant enrichment in adult ovaries. Based on CRISPR\/Cas9\u2010mediated mutagenesis, we found that\n                      <jats:italic>BdPiwi<\/jats:italic>\n                      loss caused severe gonadal arrest and complete sterility in both sexes\u2014females lacked detectable oocytes and males produced no mature sperm. Transcriptomic analysis uncovered widespread transcriptional upregulation, extensive transposon derepression, and dysregulated immunometabolic genes likely contributing to oogenic failure. Additionally, mutants exhibited prolonged larval duration and abnormal eclosion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      These findings demonstrate that\n                      <jats:italic>BdPiwi<\/jats:italic>\n                      is a pleiotropic regulator governing gonadogenesis, fertility, and metamorphic transition in\n                      <jats:italic>B. dorsalis<\/jats:italic>\n                      . This study expands the functional repertoire of\n                      <jats:italic>Piwi<\/jats:italic>\n                      genes in insect pests and provides a promising molecular target for genetic\u2010based management of this devastating invasive species. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.71378","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:36:36Z","timestamp":1791812196000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>\n                      <i>BdPiwi<\/i>\n                    <\/scp>\n                    is essential for gametogenesis and post\u2010embryonic development in the oriental fruit fly,\n                    <scp>\n                      <i>Bactrocera dorsalis<\/i>\n                    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Education Institutes, College of Plant Protection Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Awawing A","family":"Andongma","sequence":"additional","affiliation":[{"name":"Insect and Parasite Ecology Group, Lancaster Environment Centre Lancaster University  Lancaster UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1378-6765","authenticated-orcid":false,"given":"Shuai","family":"Zhan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, College of Plant Protection Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1903-1088","authenticated-orcid":false,"given":"Yaohui","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, College of Plant Protection Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1146\/annurev-genet-120417-031441","article-title":"piRNA\u2010guided genome defense: from biogenesis to silencing","volume":"52","author":"Czech B","year":"2018","journal-title":"Annu Rev Genet"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/s41576-018-0073-3","article-title":"PIWI\u2010interacting RNAs: small RNAs with big 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