{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T02:45:51Z","timestamp":1791773151998,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n The regulatory functions of ovary\u2010enriched genes in female reproduction are well characterized in model insects, but remain largely unexplored in agricultural pests such as the fall armyworm (FAW),\n Spodoptera frugiperda<\/jats:italic>\n . This study aimed to identify ovary\u2010enriched genes in FAW and characterize their roles in female reproduction.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Transcriptomic analysis identified more than 3000 genes upregulated in adult ovaries. Among these, a lipase\u2010encoding gene,\n LOC118266188<\/jats:italic>\n , exhibited the highest ovarian expression. Quantitative real\u2010time polymerase chain reaction analysis revealed that\n LOC118266188<\/jats:italic>\n transcript levels surged before eclosion and remained elevated in adults. Using CRISPR\/Cas9, we generated a homozygous\n LOC118266188<\/jats:italic>\n knockout line. Mutant females produced normal egg numbers with no obvious ovarian defects, but the egg hatching rate (32.70%) was dramatically reduced compared with wild\u2010type (87.05%). Time\u2010course observation revealed that mutant embryos were arrested between 24 and 36\u2009h post\u2010oviposition. Transcriptomic analysis showed that downregulated genes in mutant ovaries were enriched in lipid metabolism pathways.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n These findings demonstrate that\n LOC118266188<\/jats:italic>\n is essential for female fertility in FAW, likely through its association with lipid metabolism. This study provides new molecular insights into insect reproduction and identifies\n LOC118266188<\/jats:italic>\n as a potential target for genetic pest control. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71379","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T02:40:03Z","timestamp":1791772803000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An ovary\u2010enriched lipase gene,\n \n LOC118266188<\/i>\n <\/scp>\n , is required for embryonic development and female fertility in\n \n Spodoptera frugiperda<\/i>\n <\/scp>"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4523-8042","authenticated-orcid":false,"given":"Yalan","family":"Cheng","sequence":"first","affiliation":[{"name":"Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection Northwest A&F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Tian","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection Northwest A&F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanghui","family":"Cao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection Northwest A&F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xien","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection Northwest A&F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1093\/aesa\/73.6.722","article-title":"Keys and illustrations for the armyworm moths of the noctuid genus Spodoptera Guen\u00e9e from the Western Hemisphere","volume":"73","author":"Todd EL","year":"1980","journal-title":"Ann Entomol Soc Am"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0165632","article-title":"First report of outbreaks of the fall armyworm Spodoptera frugiperda (J.E. 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