{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T16:40:20Z","timestamp":1792428020037,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2027,10,19]],"date-time":"2027-10-19T00:00:00Z","timestamp":1823904000000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n The Asian citrus psyllid (\n Diaphorina citri<\/jats:italic>\n ) is the insect vector of \u2018\n Candidatus<\/jats:italic>\n Liberibacter asiaticus\u2019, the causal agent of huanglongbing (HLB), the most destructive citrus disease worldwide. Current management depends largely on repeated insecticide applications, underscoring the need for sustainable alternatives. Osmoregulation is indispensable for\n D<\/jats:italic>\n .\n citri<\/jats:italic>\n because the insect feeds exclusively on sucrose\u2010rich phloem sap and must continuously regulate carbohydrate metabolism and water balance to maintain physiological homeostasis. RNA interference (RNAi)\u2010mediated silencing of sucrose hydrolase (\n DcSuh<\/jats:italic>\n ) and aquaporin (\n DcAQP<\/jats:italic>\n ), two key components of the psyllid osmoregulatory network, significantly reduces psyllid survival. However, efficient and durable delivery systems capable of sustaining RNAi under natural plant\u2010feeding conditions remain limited.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n We developed recombinant Citrus tristeza virus (CTV) vectors that express double\u2010stranded RNA (dsRNA) targeting\n DcSuh<\/jats:italic>\n and\n DcAQP<\/jats:italic>\n , enabling continuous citrus\u2010mediated RNAi.\n D<\/jats:italic>\n .\n citri<\/jats:italic>\n reared on citrus plants infected with these engineered CTV vectors were evaluated over two successive generations to determine the long\u2010term effects of gene silencing on insect performance. Silencing either target gene significantly reduced psyllid fitness by decreasing survival, longevity, and fecundity while increasing developmental abnormalities and mortality. These effects persisted in the second generation, demonstrating sustained RNAi activity.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n This study demonstrates that CTV is an efficient and durable vehicle for plant\u2010mediated RNAi in citrus and identifies osmoregulation as a highly susceptible physiological target for management of\n D<\/jats:italic>\n .\n citri<\/jats:italic>\n . Integrating virus\u2010mediated dsRNA production with essential insect physiological targets offers a promising strategy for long\u2010term suppression of psyllid populations and a sustainable approach to reducing the spread of huanglongbing. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.71407","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T15:52:14Z","timestamp":1792425134000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineering citrus for psyllid control: virus\u2010induced silencing of osmoregulatory genes reduces survival and development of\n Diaphorina citri<\/i>"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3895-6943","authenticated-orcid":false,"given":"Nabil","family":"Killiny","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, Citrus Research and Education Center, IFAS University of Florida Lake Alfred FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahnaz","family":"Rashidi","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Citrus Research and Education Center, IFAS University of Florida Lake Alfred FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yagyaraj","family":"Joshi","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Citrus Research and Education Center, IFAS University of Florida Lake Alfred FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Turksen","family":"Shilts","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Citrus Research and Education Center, IFAS University of Florida Lake Alfred FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lamiaa M","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Citrus Research and Education Center, IFAS University of Florida Lake Alfred FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manjul","family":"Dutt","sequence":"additional","affiliation":[{"name":"Department of Horticulture Science, Citrus Research and Education Center, IFAS University of Florida Lake Alfred FL USA"},{"name":"Plant Breeding Graduate Program, University of Florida Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukasz L","family":"Stelinski","sequence":"additional","affiliation":[{"name":"Department of Entomology and Nematology, Citrus Research and Education Center, IFAS University of Florida Lake Alfred FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9956-9613","authenticated-orcid":false,"given":"Bryony C","family":"Bonning","sequence":"additional","affiliation":[{"name":"Department of Entomology and Nematology, IFAS University of Florida Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"7","article-title":"Huanglongbing: a destructive, newly\u2010emerging, century\u2010old disease of citrus","volume":"88","author":"Bov\u00e9 JM","year":"2006","journal-title":"J Plant Pathol"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/eea.12025","article-title":"Asian citrus psyllid, Diaphorina citri, vector of citrus huanglongbing disease","volume":"146","author":"Hall DG","year":"2013","journal-title":"Entomol Exp Appl"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1094\/PHYTO-05-21-0182-FI","article-title":"Made for each other: vector\u2013pathogen interfaces in the huanglongbing pathosystem","volume":"112","author":"Killiny N","year":"2022","journal-title":"Phytopathology"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1093\/jxb\/erj067","article-title":"Phloem\u2010sap feeding by animals: problems and solutions","volume":"57","author":"Douglas A","year":"2006","journal-title":"J Exp Bot"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0101830","article-title":"Collection and chemical composition of phloem sap from Citrus sinensis L. 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