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SIT relies on rearing, sterilizing and releasing large numbers of male mosquitoes that will mate with fertile wild females, thus reducing production of offspring from the target population. In this study, we investigated the effects of ionizing radiation (gamma) on male and female survival, longevity, mating behavior, and sterility of <jats:italic>Ae.\u00a0aegypti<\/jats:italic> in a dose\u2013response design. This work is a first step towards developing an operational SIT field suppression program against <jats:italic>Ae.\u00a0aegypti<\/jats:italic> in St. Augustine, Florida, USA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Exposing late\u2010stage pupae to 50\u2009Gy of radiation yielded 99% male sterility while maintaining similar survival of pupae to adult emergence, adult longevity and male mating competitiveness compared to unirradiated males. Females were completely sterilized at 30\u2009Gy, and when females were dosed with 50\u2009Gy, they had a lower incidence of blood\u2010feeding than unirradiated females.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our work suggests that an ionizing radiation dose of 50\u2009Gy should be used for future development of operational SIT in our program area because at this dose males are 99% sterile while maintaining mating competitiveness against unirradiated males. Furthermore, females that might be accidentally released with sterile males as a result of errors in sex sorting also are sterile and less likely to blood\u2010feed than unirradiated females at our 50\u2009Gy dose. \u00a9 2022 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.7303","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T23:27:25Z","timestamp":1669332445000},"page":"1175-1183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Developing the radiation\u2010based sterile insect technique (<scp>SIT<\/scp>) for controlling <i>Aedes aegypti<\/i>: identification of a sterilizing dose"],"prefix":"10.1002","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1333-6294","authenticated-orcid":false,"given":"Chao","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Entomology and Nematology University of Florida  Gainesville 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