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The biopesticide spinosad is applied as a foliar spray for control of <jats:italic>T. absoluta<\/jats:italic> and has systemic properties when applied as a soil drench to the growing medium of tomato plants. The aims of this study were to determine the: (i) instar\u2010dependent tolerance of larvae to spinosad; (ii) efficacy of spinosad drench application for the control of larvae; (iii) residual period of systemic activity of spinosad in leaves and fruit after drenching; and (iv) effect of spinosad drenching on tomato plant growth parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The estimated LC<jats:sub>50<\/jats:sub> value (Lethal Concentration at which 50% of the larvae died) differed between instars. The LC<jats:sub>50<\/jats:sub> for second\u2010instar larvae (0.41\u2009ppm) to spinosad was significantly lower than that for third\u2010 (0.64\u2009ppm) and fourth\u2010instar (0.63\u2009ppm) larvae. The LC<jats:sub>80<\/jats:sub> value (Concentration at which 80% of the larvae died) for fourth\u2010instar larvae (2.48\u2009ppm) was 2.6\u2010 and 1.7\u2010fold higher than that for the second\u2010 and third\u2010instar larvae, respectively. The spinosad concentration recorded in leaves at 25\u2009days after treatment (DAT; 0.26\u2009\u03bcg\u2009g<jats:sup>\u22121<\/jats:sup>) was significantly lower than that in leaves sampled at 3, 10 and 15\u2009DAT. High larval mortalities were, however, recorded for the duration of the experiment, which lasted 25\u2009days (equivalent to one <jats:italic>T. absoluta<\/jats:italic> generation).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Systemic spinosad effectively controlled <jats:italic>T. absoluta<\/jats:italic> larvae over a prolonged period. However, drenching this insecticide violates the recommendation of the Insecticide Resistance Action Committee to avoid treating consecutive insect generations with the same mode of action and can therefore result in the evolution of insecticide resistance. \u00a9 2023 The Authors. <jats:italic>Pest Management Science<\/jats:italic> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.7366","type":"journal-article","created":{"date-parts":[[2023,1,19]],"date-time":"2023-01-19T02:07:07Z","timestamp":1674094027000},"page":"1860-1867","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Residual activity of spinosad applied as a soil drench to tomato seedlings for control of <i>Tuta absoluta<\/i>"],"prefix":"10.1002","volume":"79","author":[{"given":"Reynardt","family":"Erasmus","sequence":"first","affiliation":[{"name":"Unit for Environmental Sciences 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