{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:47:38Z","timestamp":1790948858532,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T00:00:00Z","timestamp":1675987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013314","name":"Higher Education Discipline Innovation Project","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013314","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Zeugodacus cucurbitae<\/jats:italic> is an agricultural pest species with robust reproductive capabilities capable of causing extensive damage. The advent of novel male fertility\u2010related pest control strategies has been an area of active entomological research focused on the sterile insect technique (SIT) strategy.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>RNA\u2010sequencing analyses were conducted using 16 tissue samples from adult male <jats:italic>Z. cucurbitae<\/jats:italic>, leading to the identification of 5338 genes that were differentially expressed between the testes and three other analyzed tissue types. Of these genes, 808 exhibited high levels of testis expression. A quantitative polymerase chain reaction (qPCR) approach was used to validate the expression of ten of these genes selected at random, including <jats:italic>ZcTSSK1<\/jats:italic> and <jats:italic>ZcTSSK3<\/jats:italic>, which are testis\u2010specific serine\/threonine protein kinase (<jats:italic>TSSK<\/jats:italic>) genes. Evaluation via a loss\u2010of\u2010function\u2010based knockdown assay showed that the down\u2010regulation of either of these two genes in males was associated with significantly decreased egg hatching rates. <jats:italic>In situ<\/jats:italic> hybridization analyses revealed the expression of both of these transcripts in the transformation zone, and significant decreases in <jats:italic>Z. cucurbitae<\/jats:italic> sperm numbers were observed following double\u2010stranded RNA treatment. Together, these results suggested that inhibiting <jats:italic>ZcTSSK1<\/jats:italic> and <jats:italic>ZcTSSK3<\/jats:italic> expression was sufficient to alter male fertility in <jats:italic>Z. cucurbitae.<\/jats:italic><\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These transcriptional sequencing results provide a foundation for further efforts to clarify the regulators of <jats:italic>Z. cucurbitae<\/jats:italic> male fertility. These preliminary analyses of the functions of <jats:italic>ZcTSSK<\/jats:italic> family genes as regulators of spermatogenesis underscore their importance in the processes integral to male fecundity and their potential as targets for pest control efforts centered on the genetic manipulation of males. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.7378","type":"journal-article","created":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T21:43:41Z","timestamp":1674596621000},"page":"2040-2049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Comparative multi\u2010tissue analyses identify testis\u2010specific serine\/threonine protein kinase (<scp><i>TSSK<\/i><\/scp>) genes involved in male fertility in the melon fly <scp><i>Zeugodacus cucurbitae<\/i><\/scp>"],"prefix":"10.1002","volume":"79","author":[{"given":"Xiao\u2010Di","family":"Zhai","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection Southwest University  Chongqing China"},{"name":"International Joint Laboratory of China\u2010Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences Southwest University  Chongqing China"},{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education) Southwest University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su\u2010Yun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection Southwest University  Chongqing China"},{"name":"International Joint Laboratory of China\u2010Belgium on Sustainable Crop Pest Control, Academy of 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