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Despite these interventions, the dengue burden is increasing in Bangladesh, and the country experienced its worst outbreak in 2019 with 101\u2009354 hospitalized cases. This may be partially facilitated by the presence of intense insecticide resistance in vector populations. Here, we describe the intensity and mechanisms of resistance to insecticides commonly deployed against\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      in Dhaka, Bangladesh.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Dhaka\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      colonies exhibited high\u2010intensity resistance to pyrethroids. Using CDC bottle assays, we recorded 2\u201324% mortality (recorded at 24\u2009h) to permethrin and 48\u201394% mortality to deltamethrin, at 10\u00d7 the diagnostic dose. Bioassays conducted using insecticide\u2013synergist combinations suggested that metabolic mechanisms were contributing to pyrethroid resistance, specifically multi\u2010function oxidases, esterases, and glutathione S\u2010transferases. In addition,\n                      <jats:italic>kdr<\/jats:italic>\n                      alleles were detected, with a high frequency (78\u201398%) of homozygotes for the V1016G mutation. A large proportion (\u2264\u200974%) of free\u2010flying and resting mosquitoes from Dhaka colonies survived exposure to standard applications of pyrethroid aerosols in an experimental free\u2010flight room. Although that exposure affected the immediate host\u2010seeking behavior of\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      , the effect was transient in surviving mosquitoes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      The intense resistance characterized in this study is likely compromising the operational effectiveness of pyrethroids against\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      in Dhaka. Switching to alternative chemical classes may offer a medium\u2010term solution, but ultimately a more sustainable and effective approach to controlling dengue vectors is required. \u00a9 2023 The Authors.\n                      <jats:italic>Pest Management Science<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.7462","type":"journal-article","created":{"date-parts":[[2023,3,21]],"date-time":"2023-03-21T06:45:02Z","timestamp":1679381102000},"page":"2846-2861","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Insecticide resistance compromises the control of\n                    <i>Aedes aegypti<\/i>\n                    in Bangladesh"],"prefix":"10.1002","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2027-2399","authenticated-orcid":false,"given":"Hasan Mohammad","family":"Al\u2010Amin","sequence":"first","affiliation":[{"name":"Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute  Brisbane Queensland Australia"},{"name":"School of Biological Sciences University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narayan","family":"Gyawali","sequence":"additional","affiliation":[{"name":"Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Graham","sequence":"additional","affiliation":[{"name":"Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Shafiul","family":"Alam","sequence":"additional","affiliation":[{"name":"International Center for Diarrhoeal Disease Research, Bangladesh (icddr,b)  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Lenhart","sequence":"additional","affiliation":[{"name":"Entomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon E","family":"Hugo","sequence":"additional","affiliation":[{"name":"Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordana","family":"Ra\u0161i\u0107","sequence":"additional","affiliation":[{"name":"Mosquito Genomics, QIMR Berghofer Medical Research Institute  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel W","family":"Beebe","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of Queensland  Brisbane Queensland Australia"},{"name":"CSIRO  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregor J","family":"Devine","sequence":"additional","affiliation":[{"name":"Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"e_1_2_10_3_1","unstructured":"IEDCR Dengue Situation Reportshttps:\/\/old.iedcr.gov.bd\/website\/index.php\/surveillance\/219[accessed 8 September 2021]."},{"key":"e_1_2_10_4_1","unstructured":"Anonymous Dengue.http:\/\/dashboard.dghs.gov.bd\/webportal\/pages\/heoc_dengue.php[accessed 15.01.23 2023]."},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005475"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.42869"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003907"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006561"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009106"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apjtm.2017.08.015"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/acn3.556"},{"key":"e_1_2_10_12_1","unstructured":"AnamM Let's Take the Fight to Aedeshttps:\/\/www.thedailystar.net\/views\/editorial\/news\/lets-take-the-fight-aedes-2159071[accessed 9 September 2021]."},{"key":"e_1_2_10_13_1","unstructured":"KhanAA Dissemination workshop on monsoonAedesSurvey\u20102021 & current situation of dengue. 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