{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T08:18:37Z","timestamp":1790929117633,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,9,14]],"date-time":"2024-09-14T00:00:00Z","timestamp":1726272000000},"content-version":"am","delay-in-days":354,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2024,2]]},"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n Crop diseases caused by plant pathogenic fungi and bacteria have led to substantial losses in global food production. Chemical pesticides have been widely used as a primary means to mitigate these issues. Nevertheless, the persistent and excessive use of pesticides has resulted in the emergence of microbial resistance. Moreover, the improper application and excessive utilization of pesticides can contribute to environmental pollution and the persistence of pesticide residues. Consequently, the development of novel and highly effective bactericides and fungicides to combat plant pathogens holds immense practical importance.<\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n A series of uracil hydrazones\n IV\u2010B<\/jats:bold>\n was deliberately designed and evaluated for their antimicrobial efficacy. The results of bioassays indicated that most\n IV\u2010B<\/jats:bold>\n exhibited >80% inhibition against the fungal species\n Monilia fructigena<\/jats:italic>\n and\n Sclerotium rolfsii<\/jats:italic>\n , as well as the bacterial species\n Clavibacter michiganensis<\/jats:italic>\n subsp.\n michiganensis<\/jats:italic>\n ,\n Xanthomonas oryzae<\/jats:italic>\n pv.\n oryzae<\/jats:italic>\n , and\n Ralstonia solanacearum<\/jats:italic>\n , at 50\u2009\u03bcg\/mL\n in vitro<\/jats:italic>\n .\n In vivo<\/jats:italic>\n ,\n IV\u2010B20<\/jats:bold>\n showed 89.9% of curative and 71.8% of protective activities against\n C. michiganensis<\/jats:italic>\n subsp.\n michiganensis<\/jats:italic>\n at 100\u2009\u03bcg\/mL superior to thiodiazole copper and copper hydroxide.\n IV\u2010B20<\/jats:bold>\n also showed excellent protective activity against\n M. fructigena<\/jats:italic>\n (96.3% at 200\u2009\u03bcg\/mL) and\n S. rolfsii<\/jats:italic>\n (80.4% at 1000\u2009\u03bcg\/mL), which were greater than chlorothalonil and equivalent to thifluzamide. Mechanistic studies revealed that\n IV\u2010B20<\/jats:bold>\n induced oxidative damage in pathogenic bacteria and promoted the leakage of cellular contents.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n This study suggests that\n IV\u2010B20<\/jats:bold>\n with uracil hydrazone skeleton has great potential as an antimicrobial candidate. These findings lay a foundation for practical application in agriculture. \u00a9 2023 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.7771","type":"journal-article","created":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T14:15:39Z","timestamp":1694700939000},"page":"414-425","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Uracil hydrazones: design, synthesis, antimicrobial activities, and putative mode of action"],"prefix":"10.1002","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-8009-0994","authenticated-orcid":false,"given":"Huan","family":"Zhou","sequence":"first","affiliation":[{"name":"Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry Central China Normal University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing 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