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The most common control measure to prevent canker development is the application of fungicides which are being phased out by European Union regulations. In the present study, two sets of bacterial strains were evaluated for their antifungal activity against pathogenic <jats:italic>Botryosphaeriaceae<\/jats:italic> species through <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> antagonism assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The rhizospheric bacteria <jats:italic>Pseudomonas aeruginosa<\/jats:italic> AC17 and <jats:italic>Bacillus velezensis<\/jats:italic> ACH16, as well as the endophytic bacteria <jats:italic>Bacillus mobilis<\/jats:italic> Sol 1\u20132, respectively inhibited 87, 95, and 63% of the mycelial growth of <jats:italic>Neofusicoccum parvum<\/jats:italic>, <jats:italic>Botryosphaeria dothidea<\/jats:italic>, <jats:italic>Diplodia seriata<\/jats:italic>, and <jats:italic>Macrophomina phaseolina<\/jats:italic>. Additionally, they significantly reduced the length of lesions caused by <jats:italic>N<\/jats:italic>. <jats:italic>parvum<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>dothidea<\/jats:italic> in artificially inoculated detached almond twigs. All these bacterial strains produce hydrolytic enzymes that are able to degrade the fungal cell wall. <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic> AC17 also produces toxic volatile compounds, such as hydrogen cyanide. This strain was the most effective in controlling Botryosphaeria dieback <jats:italic>in planta<\/jats:italic> under controlled conditions at a level similar to the biocontrol agent <jats:italic>Trichoderma atroviride<\/jats:italic> and standard chemical fungicide treatments.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> AC17 is the best candidate to be considered as a potential biocontrol agent against <jats:italic>Botryosphaeriaceae<\/jats:italic> fungi affecting almond. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.7919","type":"journal-article","created":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T07:50:08Z","timestamp":1701762608000},"page":"1839-1848","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Biocontrol of almond canker diseases caused by <scp><i>Botryosphaeriaceae<\/i><\/scp> fungi"],"prefix":"10.1002","volume":"80","author":[{"given":"Laura","family":"Romero\u2010Cuadrado","sequence":"first","affiliation":[{"name":"Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA) Center Las Torres  Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Cinta","family":"Picos","sequence":"additional","affiliation":[{"name":"Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA) Center Las Torres  Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Camacho","sequence":"additional","affiliation":[{"name":"Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA) Center Las Torres  Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Javier","family":"Ollero","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Seville  Seville 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