{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T01:10:45Z","timestamp":1783041045805,"version":"3.54.6"},"reference-count":56,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T00:00:00Z","timestamp":1702598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004591","name":"Kansainv\u00e4lisen Liikkuvuuden ja Yhteisty\u00f6n Keskus","doi-asserted-by":"publisher","award":["UID\/AGR\/00690\/2020"],"award-info":[{"award-number":["UID\/AGR\/00690\/2020"]}],"id":[{"id":"10.13039\/501100004591","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012449","name":"Southern University of Science and Technology","doi-asserted-by":"publisher","award":["LA\/P\/0007\/2020"],"award-info":[{"award-number":["LA\/P\/0007\/2020"]}],"id":[{"id":"10.13039\/501100012449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["UNEX\u2010AE\u20103394"],"award-info":[{"award-number":["UNEX\u2010AE\u20103394"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["BES\u20102017\u2010081340"],"award-info":[{"award-number":["BES\u20102017\u2010081340"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Gnomoniopsis smithogilvyi<\/jats:italic> is the major chestnut pathogen, responsible for economic losses and recently described as a 3\u2010nitropropionic acid and diplodiatoxin mycotoxin producer. <jats:italic>Bacillus amyloliquefaciens<\/jats:italic> QST 713 (Serenade\u00ae ASO), <jats:italic>B. amyloliquefaciens<\/jats:italic> CIMO\u2010BCA1, and the fungicide Horizon\u00ae (tebuconazole) have been shown to reduce the growth of <jats:italic>G. smithogilvyi<\/jats:italic>. However, they enhanced mycotoxin production. Proteomics can clarify the mould's physiology and the impact of antifungal agents on the mould's metabolism. Thus, the aim of this study was to assess the impact of Horizon\u00ae, Serenade\u00ae, and <jats:italic>B. amyloliquefaciens<\/jats:italic> CIMO\u2010BCA1 in the proteome of <jats:italic>G. smithogilvyi<\/jats:italic> to unveil their modes of action and decipher why the mould responds by increasing the mycotoxin production. For this, the mycelium close to the inhibition zone provoked by antifungals was macroscopically and microscopically observed. Proteins were extracted and analysed using a Q\u2010Exactive plus Orbitrap.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The results did not elucidate specific proteins involved in the mycotoxin biosynthesis, but these agents provoked different kinds of stress on the mould, mainly affecting the cell wall structures and antioxidant response, which points to the mycotoxins overproduction as a defence mechanism. The biocontrol agent CIMO\u2010BCA1 acts similar to tebuconazole. The results revealed different responses on the mould's metabolism when co\u2010cultured with the two <jats:italic>B. amyloliquefaciens<\/jats:italic>, showing different modes of action of each bacterium, which opens the possibility of combining both biocontrol strategies.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These results unveil different modes of action of the treatments that could help to reduce the use of toxic chemicals to combat plant pathogens worldwide. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.7920","type":"journal-article","created":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T02:58:39Z","timestamp":1701831519000},"page":"1895-1903","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Unravelling the effect of control agents on <scp><i>Gnomoniopsis smithogilvyi<\/i><\/scp> on a chestnut\u2010based medium by proteomics"],"prefix":"10.1002","volume":"80","author":[{"given":"Micaela","family":"\u00c1lvarez","sequence":"first","affiliation":[{"name":"Higiene y Seguridad Alimentaria, Instituto Universitario de Investigaci\u00f3n de Carne y Productos C\u00e1rnicos, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"},{"name":"Secci\u00f3n Departamental de Nutrici\u00f3n y Ciencia de los Alimentos (Nutrici\u00f3n, Bromatolog\u00eda, Higiene y Seguridad Alimentaria), Facultad de Veterinaria Universidad Complutense de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isadora","family":"Agostini","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia  Bragan\u00e7a Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro\u2010Environmental and Biological Sciences University of Tr\u00e1s\u2010os\u2010Montes e Alto Douro (UTAD)  Vila Real Portugal"},{"name":"Laborat\u00f3rio Associado Instituto para a Inova\u00e7\u00e3o, Capacita\u00e7\u00e3o e Sustentabilidade da Produ\u00e7\u00e3o Agroalimentar, Universidad de Tr\u00e1s\u2010os\u2010Montes e Alto Douro (UTAD), Quinta de Prados  Vila Real Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ngel","family":"Rom\u00e1n","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Biolog\u00eda Molecular y Gen\u00e9tica, Facultad de Ciencias Universidad de Extremadura  Badajoz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4074-981X","authenticated-orcid":false,"given":"Josu\u00e9","family":"Delgado","sequence":"additional","affiliation":[{"name":"Higiene y Seguridad Alimentaria, Instituto Universitario de Investigaci\u00f3n de Carne y Productos C\u00e1rnicos, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3789-2730","authenticated-orcid":false,"given":"Paula","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia  Bragan\u00e7a Portugal"},{"name":"Laborat\u00f3rio Associado para a Sustentabilidade e Tecnologia em Regi\u00f5es de Montanha, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia  Bragan\u00e7a Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof9040401"},{"key":"e_1_2_8_3_1","first-page":"1166","article-title":"Mycotoxins and other secondary metabolites are produced by Gnomoniopsis smithogilvyi when confronted with biological and chemical control agents","volume":"13","author":"\u00c1lvarez M","year":"2023","journal-title":"Agri"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.4259"},{"key":"e_1_2_8_5_1","first-page":"26","article-title":"Cytotoxicity of diplodiatoxin, dipmatol and diplonine, metabolites synthesized by Stenocarpella maydi","volume":"82","author":"Masango MG","year":"2014","journal-title":"Toxin"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1586\/eri.09.67"},{"key":"e_1_2_8_7_1","first-page":"234","article-title":"Exposure to the agricultural fungicide tebuconazole promotes Aspergillus fumigatus cross\u2010resistance to clinical azoles","volume":"44","author":"Allizond V","year":"2021","journal-title":"New Microbiol"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-14-155"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42483-020-00064-9"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-021-02353-1"},{"key":"e_1_2_8_11_1","volume-title":"Microbial Bioprotectants for Plant Disease Management","author":"Anckaert A","year":"2022"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2019.104067"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jph.12455"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2019.104158"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2016.02.011"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2019.03.013"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.12657\/denbio.075.011"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2020.107222"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2007.08.008"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2016.10.031"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24054709"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2021.108695"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2022.109858"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.11140"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2019.108243"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1511"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1052"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp101"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.10.142"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology11101390"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.5941\/MYCO.2013.41.4.234"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111108"},{"key":"e_1_2_8_33_1","first-page":"1","article-title":"Tetrahydrobiopterin plays a functionally significant role in lipogenesis in the oleaginous fungus Mortierella alpina","volume":"11","author":"Wang H","year":"2020","journal-title":"Front Microbiol"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2018.09.057"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2006.00496.x"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2013.05.009"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01234"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2014.09.008"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02993"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.10.009"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11425.x"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-014-0455-9"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2019.1682760"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmpp.2021.101675"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02986-19"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06178.x"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01794"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0048-3575(90)90132-L"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704298200"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2003.09.004"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01291-18"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2410.180730"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2021.103741"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2019.103266"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2023.132021"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins13010051"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2019.108265"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.7920","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T09:34:10Z","timestamp":1761039250000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.7920"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,15]]},"references-count":56,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/ps.7920"],"URL":"https:\/\/doi.org\/10.1002\/ps.7920","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,15]]},"assertion":[{"value":"2023-10-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}