{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:45:59Z","timestamp":1790873159366,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T00:00:00Z","timestamp":1703808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072461"],"award-info":[{"award-number":["32072461"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Sclerotinia stem rot caused by <jats:italic>Sclerotinia sclerotiorum<\/jats:italic> seriously endangers oilseed rape production worldwide, and the occurrence of fungicide\u2010resistant mutants of <jats:italic>S. sclerotiorum<\/jats:italic> leads to control decline. Thus, it is critical to explore new green substitutes with different action mechanisms and high antifungal activity. Herein, the activity and the action mechanism of natamycin against <jats:italic>S. sclerotiorum<\/jats:italic> were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Natamycin showed potent inhibition on the mycelial growth of <jats:italic>S. sclerotiorum<\/jats:italic>, and half\u2010maximal effective concentration (EC<jats:sub>50<\/jats:sub>) values against 103 <jats:italic>S. sclerotiorum<\/jats:italic> strains ranged from 0.53 to 4.04\u2009\u03bcg\/mL (mean 1.44\u2009\u03bcg\/mL). Natamycin also exhibited high efficacy against both carbendazim\u2010 and dimethachlone\u2010resistant strains of <jats:italic>S. sclerotiorum<\/jats:italic> on detached oilseed rape leaves. No cross\u2010resistance was detected between natamycin and carbendazim. Natamycin markedly disrupted hyphal form, sclerotia formation, integrity of the cell membrane, and reduced the content of oxalic acid and ergosterol, whereas it increased the reactive oxygen species (ROS) and malondialdehyde content. Interestingly, exogenous addition of ergosterol could reduce the inhibition of natamycin against <jats:italic>S. sclerotiorum<\/jats:italic>. Importantly, natamycin significantly inhibited expression of the <jats:italic>Cyp51<\/jats:italic> gene, which is contrary to results for the triazole fungicide flusilazole, indicating a different action mechanism from triazole fungicides.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Natamycin is a promising effective candidate for the resistance management of <jats:italic>S. sclerotiorum<\/jats:italic>. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.7930","type":"journal-article","created":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T23:45:51Z","timestamp":1702424751000},"page":"1981-1990","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Efficacy and toxic action of the natural product natamycin against <scp><i>Sclerotinia 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