{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T14:27:36Z","timestamp":1789741656872,"version":"4.0.1"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Resistance problems with the long\u2010term and frequent use of existing fungicides, and the lack of structure diversity of traditional pyrazole\u20104\u2010carboxamide succinate dehydrogenase inhibitors, it is highly required to design and develop new fungicides to address the resistance issue.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Different from previous pyrazole\u20104\u2010carboxamide succinate dehydrogenase inhibitors by breaking the norm of difluoromethyl at the C\u20103 position of pyrazole and introducing a tertiary alcohol group at the C\u20103 position, 27 novel pyrazole\u20104\u2010carboxamide derivatives were designed, synthesized and characterized by proton (<jats:sup>1<\/jats:sup>H) nuclear magnetic resonance (NMR), carbon\u201013 (<jats:sup>13<\/jats:sup>C) NMR, fluorine\u201019 (<jats:sup>19<\/jats:sup>F) NMR and high\u2010resolution electrospray ionization mass spectrometry (HR\u2010ESI\u2010MS). The crystal structures of compounds A14 and C5 were analyzed by single crystal X\u2010ray diffraction. Their <jats:italic>in vitro<\/jats:italic> antifungal activities were evaluated against phytopathogen <jats:italic>Fusarium graminearum<\/jats:italic>, <jats:italic>Botrytis cinerea<\/jats:italic>, <jats:italic>Phytophthora capsica<\/jats:italic>, <jats:italic>Sclerotinia sclerotiorum<\/jats:italic>, <jats:italic>Thanatephorus cucumeris<\/jats:italic>. The results displayed that most of them exhibited significant antifungal activities against <jats:italic>S<\/jats:italic>. <jats:italic>sclerotiorum<\/jats:italic> at 50\u2009mg\/L, the half maximal effective concentration (EC<jats:sub>50<\/jats:sub>) data of A8 and A14 were 3.96 and 2.52\u2009mg\/L, respectively. Their <jats:italic>in vivo<\/jats:italic> antifungal activities were evaluated against <jats:italic>Pseudoperonospora cubensis<\/jats:italic>, <jats:italic>Puccinia sorghi<\/jats:italic> Schw, <jats:italic>Colletotrichum gloeosporioides<\/jats:italic>, <jats:italic>F<\/jats:italic>. <jats:italic>graminearum<\/jats:italic>, <jats:italic>Erysiphe graminis<\/jats:italic>, <jats:italic>Thanatephorus cucumeris<\/jats:italic>, the control efficacies of A6, B3, C3, and C6 against <jats:italic>E<\/jats:italic>. <jats:italic>graminis<\/jats:italic> reached 100% at a concentration of 400\u2009mg\/L. The molecular docking results showed that the binding mode of the target compounds containing tertiary alcohols were similar to that of fluxapyroxad in succinate dehydrogenase. In addition, tertiary alcohols were involved in the formation of hydrogen bonds.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The excellent <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> inhibitory activities of novel pyrazole\u20104\u2010carboxamide derivatives against succinate dehydrogenase were reported for the first time, and they could be used as the potential lead compounds. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.7937","type":"journal-article","created":{"date-parts":[[2023,12,17]],"date-time":"2023-12-17T22:23:45Z","timestamp":1702851825000},"page":"2032-2041","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Design, synthesis, antifungal activity and molecular docking of novel pyrazole\u20104\u2010carboxamides containing tertiary alcohol and difluoromethyl moiety as potential succinate dehydrogenase 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