{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T18:00:11Z","timestamp":1782410411670,"version":"3.54.5"},"reference-count":65,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T00:00:00Z","timestamp":1705881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Plutella xylostella<\/jats:italic> (Linnaeus) is a destructive pest of cruciferous crops due to its strong reproductive capacity and extensive resistance to pesticides. Seminal fluid proteins (SFPs) are the main effective factors that determine the reproductive physiology and behaviour of both sexes. Although an increasing number of SFPs have been identified, the effects of astacins in SFPs on agricultural pests have not yet been reported. Here, we elucidated the mechanisms by which <jats:italic>Sast1<\/jats:italic> (seminal astacin 1) regulates the fertility of <jats:italic>Plutella xylostella<\/jats:italic> (L.).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:italic>PxSast1<\/jats:italic> was specifically expressed in the testis and accesssory gland. CRISPR\/Cas9\u2010induced <jats:italic>PxSast1<\/jats:italic> knockout successfully constructed two homozygous mutant strains. <jats:italic>Sast1<\/jats:italic> impaired the fertility of <jats:italic>P. xylostella<\/jats:italic> by separately regulating the reproductive capacity of males and females. Loss of <jats:italic>PxSast1<\/jats:italic>, on the one hand, significantly decreased the ability of males to mate and fertilize, mainly manifested as shortened mating duration, reduced mating competitiveness and decreased eupyrene sperm production; on the other hand, it significantly inhibited the expression of chorion genes in females, resulting in oogenesis deficits. Simultaneously, for mated females, the differentially expressed genes in signalling pathways related to oogenesis and chorion formation were significantly enriched after <jats:italic>PxSast1<\/jats:italic> knockout.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These analyses of the functions of <jats:italic>PxS<\/jats:italic>ast1 as the regulator of spermatogenesis and oogenesis establish its importance in the fertility process of <jats:italic>P. xylostella<\/jats:italic>, as well as its potential as a promising target for genetic regulation\u2010based pest control. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.7966","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T13:48:59Z","timestamp":1705931339000},"page":"2596-2609","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<scp><i>Sast1<\/i><\/scp>\u2010mediated manifold effects inhibit <scp><i>Plutella xylostella<\/i><\/scp> fertility"],"prefix":"10.1002","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2730-6354","authenticated-orcid":false,"given":"Min\u2010Hui","family":"Cao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Ecological Pest Control for Fujian\u2010Taiwan Crops, Institute of Applied Ecology Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross\u2010Strait Crops Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture  Fuzhou China"},{"name":"Fujian Provincial Key Laboratory of Insect Ecology Fujian Agriculture and Forestry University  Fuzhou 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