{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T08:14:29Z","timestamp":1788423269758,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T00:00:00Z","timestamp":1710460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007605","name":"Agricultural Biotechnology Research Institute of Iran","doi-asserted-by":"publisher","award":["3\u201005\u20100516\u2010017\u2010971027"],"award-info":[{"award-number":["3\u201005\u20100516\u2010017\u2010971027"]}],"id":[{"id":"10.13039\/501100007605","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The widespread use of chemical herbicides and the growing issue of weed resistance pose significant challenges in agriculture. To address these problems, there is a pressing need to develop biological herbicides based on bacterial metabolites.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In this study, we investigated the impact of the cell\u2010free culture filtrate (CFCF) from the ZT isolate, a bacilliform bacterium obtained from diseased wheat seeds, on the germination and seedling growth of various plant species, including wild oat, ryegrass, redroot, wheat, and chickpea. The results revealed that CFCF had a detrimental effect on the fresh and dry weight of stems and roots in most of the studied plants, except chickpeas. The CFCF was further subjected to separation into aqueous and organic phases using chloroform, followed by the division of the aqueous phase into 13 fractions using an alumina column. Notably, both the aqueous phase (20%) and all 13 fractions (ranging from 50% to 83%) displayed the ability to reduce the root length of ryegrass, a monocotyledonous weed. Liquid chromatography\u2013mass spectrometry (LC\u2013MS) analysis identified that fractions 3 and 7, which were effective against ryegrass but not redroot, contained Cry family proteins, including Cry10 Aa, Cry4 Ba, and Cry4 Aa. Additionally, <jats:italic>16s rRNA<\/jats:italic> gene sequencing revealed that the ZT isolate is closely related (98.27%) to <jats:italic>Bacillus wiedmannii<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Conclusively, metabolites from the ZT bacterium hold promise for monocotyledonous weed\u2010targeted herbicides, providing a constructive strategy to confront agricultural issues tied to chemical herbicides and weed resistance. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8053","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T06:30:12Z","timestamp":1709274612000},"page":"3478-3490","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Herbicidal proteins from <i>Bacillus wiedmannii<\/i> isolate <scp>ZT<\/scp> selectively inhibit ryegrass (<i>Lolium temulentum<\/i> L.)"],"prefix":"10.1002","volume":"80","author":[{"given":"Mahsa","family":"Eigharlou","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Biological Sciences Alzahra University  Tehran Iran"},{"name":"Department of Microbiology, School of Biology and Pharmaceutical Biotechnology Lab, College of Science Tehran University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeinabalsadat","family":"Hashemi","sequence":"additional","affiliation":[{"name":"Department of Microbial Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII) Agricultural Research, Education and Extension Organization (AREEO)  Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biological Sciences Alzahra University  Tehran 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