{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:17:27Z","timestamp":1772993847188,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070501"],"award-info":[{"award-number":["32070501"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2024,8]]},"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n Actin\u2010related protein 2\/3 complex regulates actin polymerization and the formation of branched actin networks. However, the function and evolutionary relationship of this complex subunit 2 (Arpc2) has been poorly understood in insects.<\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n To address these issues, we performed comprehensive analysis of Arpc2 in\n Tribolium castaneum<\/jats:italic>\n . Phylogenetic analysis revealed that\n Arpc2<\/jats:italic>\n was originated from one ancestral gene in animals but evolved independently between vertebrates and insects after species differentiation.\n T. castaneum Arpc2<\/jats:italic>\n has a 906\u2010bp coding sequence and consists of 4 exons.\n Arpc2<\/jats:italic>\n transcripts were abundantly detected in embryos and pupae but less so in larvae and adults, while it had high expression in the gut, fat body and head but low expression in the epidermis of late\u2010stage larvae. Knockdown of it at the late larval stage inhibited the pupation and resulted in arrested larvae. Silencing it in 1\u2010day pupae impaired eclosion, which caused adult wings to fail to close. Injection of\n Arpc2<\/jats:italic>\n dsRNAs into 5\u2010day pupae made adults have smaller testis and ovary and could not lay eggs. The expression of\n vitellogenin 1<\/jats:italic>\n (\n Vg1<\/jats:italic>\n ),\n Vg2<\/jats:italic>\n and\n Vg receptor<\/jats:italic>\n (\n VgR<\/jats:italic>\n ) was downregulated after knocking down\n Arpc2<\/jats:italic>\n 5\u2009days post\u2010adult emergence.\n Arpc2<\/jats:italic>\n silencing reduced 20\u2010hydroxyecdysone titer by affecting the enzymes of its biosynthesis and catabolism but increased juvenile biosynthesis via upregulating\n JHAMT3<\/jats:italic>\n expression.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n Our results indicate that Arpc2 is associated with the metamorphosis and reproduction by integrating ecdysone and juvenile hormone metabolism in\n T. castaneum<\/jats:italic>\n . This study provides theoretical basis for developing Arpc2 as a potential RNA interference target for pest control. \u00a9 2024 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.8076","type":"journal-article","created":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T07:20:26Z","timestamp":1710314426000},"page":"3734-3742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Arpc2 integrates ecdysone and juvenile hormone metabolism to influence metamorphosis and reproduction in\n \n Tribolium castaneum<\/i>\n <\/scp>"],"prefix":"10.1002","volume":"80","author":[{"given":"Runting","family":"Ge","sequence":"first","affiliation":[{"name":"School of Life Sciences Jiangsu University Zhenjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Jiangsu University Zhenjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhua","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Jiangsu University Zhenjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keping","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences Jiangsu University Zhenjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4553-0721","authenticated-orcid":false,"given":"Chengjun","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences Jiangsu University Zhenjiang China"},{"name":"Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.70.1.649"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(02)00396-2"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.261419298"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C100476200"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1130513100"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0020091"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1443"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3492"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800783200"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.221"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018392"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2017.08.003"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-08357-4"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.131382"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2017006973"},{"key":"e_1_2_9_17_1","first-page":"45","article-title":"Silencing of the ARP2\/3 complex disturbs pancreatic cancer cell migration","volume":"33","author":"Rauhala HE","year":"2013","journal-title":"Anticancer Res"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2019.01.017"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14205"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-022-10338-3"},{"key":"e_1_2_9_21_1","first-page":"3189","article-title":"ARPC2 promotes breast cancer proliferation and metastasis","volume":"41","author":"Cheng Z","year":"2019","journal-title":"Oncol Rep"},{"key":"e_1_2_9_22_1","first-page":"1387","article-title":"RBM3 upregulates ARPC2 by binding the 3'UTR and contributes to breast cancer progression","volume":"54","author":"Chen P","year":"2019","journal-title":"Int J Oncol"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02393-20"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyab175"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.033712"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.037218"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2014.03.006"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1506645112"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1359"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2020.104672"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2008.09.002"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/83.5.1745"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2009.08.005"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12327"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2012.12.014"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12938"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.120883"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0093768"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2014.08.007"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.09.023"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2010.10.021"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703719104"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2008.06428.x"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2010.04.002"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/ens.12086"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2010.03.006"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.00946"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.00679"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12817"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12107"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.8076","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.8076","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.8076","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:58:01Z","timestamp":1772981881000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.8076"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,22]]},"references-count":50,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1002\/ps.8076"],"URL":"https:\/\/doi.org\/10.1002\/ps.8076","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,22]]},"assertion":[{"value":"2023-12-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}